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University of Gothenburg
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Light Microscopy

At CCI you will find a wide range of equipment and techniques for advanced light microscopy, with the focus on fluorescence imaging. We provide expertise and know-how so that the users can chose the correct techniques for their scientific questions, perform the imaging in an efficient way and aquire data which can be further analyzed using different software applications.

Widefield Fluorescence microscopes

A widefield fluorescence microscope uses a lamp, e.g. a Mercury arc lamp, to illuminate and excite the specimen. This is a fast and economical way to obtain fluorescent images, which can be viewed directly with your eyes through the ocular or captured with a camera.

Thin specimens that do not require confocal imaging might be better analyzed using a conventional widefield microscope as it offers unsurpassed signal to noise.

Laser scanning confocal microscopes

The advantage of a confocal microscope is that it only collects the light reflected or emitted by a single plane of the specimen. This makes the images from thick specimens much sharper than with a conventional widefield fluorescent microscope, and by collecting images from several focal planes, you can reconstruct a 3D representation of your fluorescent specimen. With a confocal laser scanning microscope (LSM) it is possibile to zoom in on small details, to perform multi-color imaging and to make time series.

The possibility to select certain regions of interest (ROI) in the confocal images makes it possible to measure dynamics and interactions with FRAP and FRET.

Multiphoton microscope

Multiphoton (MP) microscopy utilizes a non-linear excitation process, usually two-photon excitation, which occurs only at the focal point of the microscope. This gives inherent optical sectioning capabilities, without cutting off out-of-focus emission, and minimizes the photobleaching and photodamage that are the ultimate limiting factors in imaging live cells. The low energy/ long wavelenght infrared (IR) excitation light is less harmful to living species than the light range used for confocal microscopy. The IR light also undergoes less scattering, which results in less background and longer penetration depths. These advantages allows investigations on thick living tissue specimens that would not otherwise be possible with conventional imaging techniques. 

In addition to multiphoton excitation of fluorophores, it is also possible to perform other non-linear microscopy techniques, like second harmonic generation (SHG).

High content screening microscope

High Content Screening (HCS) microscopy is based on a highly automated imaging system combined with software for processing and analyzing large amounts of data from fixed or living cells.

The image analysis software can generate numeric data from the images for many different parameters, such as cell number and fluorescence intensities in labeled cell constituents. Unlike flow cytometry, which measures just the overall fluorescence of each cell, the strength of HCS image analysis is its ability to recognize and quantify cell morphology, localization, movement, structures and organization within the cells.

Super-resolution microscopes

Superresolution microscopy is the common name of the different fluorescence-based microscopy techniques, which has a resolution beyond the diffraction limit of 200 nm in the lateral direction and 500 nm in the axial direction.

CCI offers three such superresolution techniques: the AiryScan, with up to 1.7x resolution enhancement, the structured illumination microscopy (SIM), which doubles the resolution in all directions, and the single molecule localization techniques (PALM/dSTORM), which have a resolution approaching electron microscopy (down to 15 nm).

Laser microdissection microscope

The Laser Microdissection and Pressure Catapulting (LMPC) technology offers non-contact sample handling without mechanical contact of specific tissue regions for downstream analysis of RNA, DNA and proteins without risk of contamination or infection. In addition, living cells can be catapulted for subsequent recultivation of specific clones of e.g. cells expressing fluorescent proteins.

Sample preparation - cryostat

The Leica CM1950 is a cryostat with an encapsulated microtome and separate specimen cooling. The displays and instrument controls are easy to operate due to their largely self-explanatory symbols. LED displays make it easy to read. The cryochamber is made of seamlessly welded, high-quality stainless steel that is free of difficult-to-access corners and thus easy to clean and disinfect. 

This cryostat is primarily intended for the users of the laser microdissection system. Other users are also welcome if they keep the necessary cleanliness level and respect that the cryostat has to be absolutely RNAse free.

You have to bring all consumables you need for using the cryostat, e.g. the disposable blades, OCT, cryofect etc. We provide specimen discs but if you want to use the specimen discs outside the facility (i.e at EBM or at your lab) you have to buy your own. For information about the consumables please contact us.

At the CCI we give courses in light and electron microscopy as well as image analysis. However, to get access to our equipment you need to get an individual training at the particular microscope or sample preparation equipment. These trainings are set up after a face-to-face project meeting. 

The individual introductory microscope training usually consists of two sessions, one half day of instruction (3 hours) with a training specimen and one half day with the user’s own specimen, but can be shorter or  extended depending on the microscope technique and the needs and experience of the user.

Please contact the CCI staff, preferably using cci@gu.se, for more information about prices and to schedule the training sessions.