LSM 880 Airyscan laser module is equipped with 4 Lasers:
Diode laser 405 nm
Argon-laser 458, 488, 514 nm
DPSS-laser 561 nm
HeNe-laser 633 nm
Plan-Apochromat 10x/0.45 air
Plan-Apochromat 20x/0.8 air
Plan-Apochromat 40x/1.4 oil immersion
Plan-Apochromat 63x/1.4 oil immersion
C-Apochromat 40x/1.2 water immersion
The Airyscan module has the following filter combinations:
BP 420-480 + BP 495-550
BP 420-480 + BP 495-620
BP 420-480 + LP 605
BP 465-505 + LP 525
BP 495-550 + LP 570
BP 570-620 + LP 645
Camera for widefield imaging
The Hamamatsu Orca Flash 4.0 V2 digital CMOS sensor has low readout noise as compared to the conventional cooling CCD image sensor, a high-speed readout of 100 frames/second at 4 megapixels (2048x2048 pixels with a size of 6.5 μm x 6.5 μm) and a peak QE of 82%.
The confocal and Airyscan imaging is performed using the Zen Black 2.3 software, which includes the "Experiment Designer" module for setting up advanced time-course experiments in blocks with different acquisition parameters. The widefield imaging is performed using the Zen Blue 2.3 software.
Advanced 3D/4D rendering of big data, powering by the "Arivis" algorithm, is possible with the Zen Blue 2.3 software.