Laser lines for confocal imaging
- Laser diode 405 nm (25 mW)
- Ar-laser 458, 488, 514 nm (25 mW)
- Laser diode 561nm
- HeNe-laser 633 nm (5 mW)
Laser for multiphoton imaging
The InSight DeepSee from Spectra-Physics is our ultrafast laser system that takes multiphoton imaging to new depths! This femtosecond pulsed laser has a wide tunable range between 680 nm to 1300 nm. There is an integrated dispersion pre-compensator, which makes sure that short pulses reach the sample for maximum fluorescence. A second output beam, with a fixed wavelength at 1040 nm, complements the main tuneable output, is available for advanced imaging techniques including uncaging, CARS imaging and multimodal imaging.
Non-Descanned Detectors (NDDs)
Fluorescence light excited by a multiphoton (non-linear) process originates only from the focus. Thus, no confocal pinhole is needed, and the signal can be projected directly from the specimen onto the detector. Our LSM 710 NLO has four standard non-descanned detection channels for reflection mode and two for transmission. The installed emission filters are SP 485 for blue fluorophores, BP 500-550 for green fluorophores, BP 560-610 for orange-red fluorophores, and BP 640-710 for far-red fluorophores.
GaAsP Non-Descanned Detector
The challenge of intra-vital imaging is the need to visualize very fine details in tissue that may be extremely light-scattering. The GaAsP Non-descanned detector with its high efficiency detection and low dark noise is the ideal tool for such tasks. This detector not only increases the flexibility but provides an up to 2-fold better signal-to-noise ratio and correspondingly a higher sensitivity.