LSM980 with Airyscan 2

Built for navigating and correlating with ease in complex samples. Multicolour images can be acquired with the Widefield system and then used to manual or automated navigation into the sample for more detail confocal studies. This workflow is also improved by the use of the ZEN connect and Guided Acquisition software.

A light-efficient beam path with up to 34 simultaneous channels and full spectral flexibility results in a great confocal for multi-color experiments with living samples. The unique combination of spectral imaging with improved signal-to-noise ratio and resolution enables lower laser power for your live cell experiments.

To separate even highly overlapping signals or to remove autofluorescence, the Lambda Scan can be used to measure up to 32 distinct spectral channel with an Spectral resolution of 8.9 nm. Then spectral unmixing can be used to recover the occurrence of each fluorophore. This can be done using ZEN blue or home built algorithms developed at CCI. 

Airyscan 2 is an area detector with 32 honeycomb arranged detection elements. Due to their physical size and the use of a telescope in the beam path, each element acts as a pinhole, contributing to super-resolution information, while the complete detector area collects more light than the standard confocal setting.

In Multiplex modes, Airyscan detector advantages are combined with adapted illumination and readout schemes, giving different choices for parallelization (faster imaging speeds).

WF Transmission

• Halogen Lamp

WF Fluorescence LEDs Colibri

• 385 nm
• 475 nm
• 555 nm
• 630 nm

WF Dichroic filter

QUAD filterset:
Excitation: 385 nm (370-400) 475 nm (450-488) 555 nm (540-570) & 630 nm (614-647)

Emission: BP 410-440 nm, BP 499-529 nm, BP 579-604 nm & BP 659-759 nm

Camera:  Axiocam 705 mono, 5 megapixel monochrome CMOS camera, 3.45 μm pixel size. 60 frames per second with full 5 megapixels or faster after cropping. Peak Quantum efficiency of up to 72 % and a broad spectral sensitivity ranging from UV to near-IR light. 

Lasers

• 405 nm
• 488 nm
• 561 nm
• 639 nm

2 x PMT Multialkaline Detectors

32-channel GaAsp Detector

Airyscan 2 GaAsP detector: is an area detector with 32 honeycomb arranged detection elements. Due to their physical size and the use of a telescope in the beam path, each element acts as a pinhole, contributing to super-resolution information, while the complete detector area collects more light than the standard confocal setting.

• BP 420-480 nm + BP 495-550 nm
• BP 420-480 nm + BP 570-630 nm
• BP 420-500 nm + LP 605 nm
• BP 465-505 nm + BP 525-585 nm
• BP 495-550 nm + BP 570-630 nm
• BP 495-555 nm + LP 660 nm
• BP 570-620 nm + LP 660 nm