University of Gothenburg
Elyra 7 Lattice SIM
Photo: Rafael Camacho CCI

ELYRA 7 with Lattice SIM and SMLM

The Carl Zeiss ELYRA7 is a state-of-the-art inverted widefield microscope designed for fast (Super-resolution) live-cell imaging via Lattice Structured Illumination Microscopy (SIM) and single-molecule localization microscopy (SMLM).

The Elyra 7 microscope

The ELYRA 7 microscope is a widefield inverted microscope designed for high-speed super-resolution imaging of biological specimens with minimal light exposure. It is equipped with an incubation system (temperature and CO2 control) and a dual camera adapter that houses two sCMOS cameras (PCO edge) for imaging two channels simultaneously.

The ELYRA7 is capable of out-of-focus light suppression and sectioning via the Apotome module and doubling the resolution of conventional widefield via Lattice Structured Illumination Microscopy (SIM). Novel image processing and imaging modalities like Burst and Leap modes can be used for increased speed and efficiency during imaging. Further, the ELYRA7 also allows for Single Molecule Localization Microscopy (SMLM) techniques such as PALM, dSTORM, and PAINT. SMLM is available with high-power lasers across the visible spectrum giving access to a broad range of fluorescent markers.

Elyra 7 micrsocope
Photo: ZEISS
Light pattern example
Photo: Rafael Camacho CCI

Imaging modalities of the ELYRA 7

Photo: Rafael Camacho CCI
Photo: Rafael Camacho CCI
Photo: Rafael Camacho CCI

Elyra 7 system characteristics

Objective lenses

Air objectives (Apotome)

Immersion objectives (SIM, SMLM)

Light sources


  • HR Diode 405 nm 50 mW
  • HR Diode 488 nm 500 mW
  • HR Diode 561 nm 500 mW
  • HR Diode 642 nm 500 mW

Fluorescent Lamp

  • HXP fluorescent lamp

Note: Elyra 7 is capable of TIRF illumination for laser widefield and SMLM


Reflector module:

Filterset 1: Flexible

  • LBF 405/488/561/642 (QUAD)
  • Excitation 405/488/642 Emission BP 420-480 + BP 495-550 + LP 655
  • Excitation 488/561 Emission BP 495-550 + BP 570-620
  • Excitation 405/642 Emission BP 420-480 + LP 655

Filterset 2: Pure

  • LBF 405/488/561/642 (QUAD)
  • Excitation 405 Emission BP 420-480
  • Excitation 488 Emission EF BP 495-590
  • Excitation 561 Emission EF LP 570


  • SR Quad: Position 1 LP 560 | Position 2 BP 490-560 + LP 640
  • SR DUO: Position 1 LP 560 | Position 2 LP 640


Duolink adapter to operate two sCMOS cameras:

  • pco.edge 4.2 CL HS sCMOS camera, 6.5 µm pixel size, peak QE 82%, liquid cooled