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Program & Speakers

The workshop will take place from November 29th to December 1st. The lectures will take place at the Wallenberg Centre, located at Medicinareberget, above the Sahlgrenska Hospital in Gothenburg. The hands-on sessions will take place in the Centre for Cellular Imaging, Core Facilities, at Medicinareberget.

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CAT Workshop 2023

Infrastructure at Core Facilities

Program - Lectures

Venue: Wallenberg Centre

November 29th

Time	Event
8:00-8:45	Registration
8:45-9:00	Welcome from the Head of the Centre for Cellular Imaging, Julia Fernandez-Rodriguez
Session 1	Introduction to Array Tomography
9:00-9:30	Jemima Burden, Head of Electron Microscopy, MRC Laboratory for Molecular and Cell Biology, University College London, United Kingdom Title: The What, Why and When of Array Tomography
9:30-10:00	Rafael Camacho, Scientific Officer in Volume Electron Microscopy at the Centre for Cellular Imaging, University of Gothenburg, Sweden Title: Correlative Array Tomography at the Centre for Cellular Imaging
10:00-10:02	Rebecca Howard, SciLifeLab, Cell and Molecular Imaging platform Flash-talk: The Cell and Molecular Imaging platform
10:02-10:30	Coffee-break
10:30-11:00	Group discussions
Session 2	Cellular 3D Volume - Array Tomography
11:00-11:30	Irene Wacker, Cryo EM, AG Schröder & BioQuant, Universität Heidelberg, Germany Title: From killer cells to design materials - 10 years AT off the beaten track
11:30-12:00	Luke Noon, Principal Investigator, Center for Investigation Principe Felipe (CIPF), Valencia, Spain Title: Connecting hepatic nerves: taking 3D-connectomics to the PNS using correlative volume electron microscopy
12:00-13:00	Lunch
Session 3	Large 3D Volumes - Array tomography
13:00-13:30	Jacob Hoogenboom, Associate professor, Department of Imaging Physics, Integrated Light and Electron Microscopy, Delft University of Technology, Netherlands Title: Faster SEM for large-scale imaging
13:30-14:00	Richard Schalek, Research Scientist Associate at Harvard University, United States Title: Realizing the benefits and opportunities of generating large-scale volume EM datasets by solving some inherent workflow challenges
14:00-14:20	DELMIC, Katherine Lau, Company Presentation Title: FAST-EM – Obtain powerful insights from your biological samples through ultrafast nano imaging.
14:20-15:00	Coffee-break
Session 4	Correlative Array Tomography
15:00-15:30 Remote	Martina Schifferer, Associate Investigator Electron Microscopy Hub, DZNE - German Center for Neurodegenerative Diseases, Munich, Germany Title: Correlative ATUM approaches to study neurodegeneration
15:30-15:50	THERMO FISHER, Richard Vernon, Company Presentation Highly efficient Array Tomography workflow: Increased precision with reduced manual interventions
15:50-16:10	LEICA, Andreas Nowak, Company Presentation Leica ARTOS 3D - Array Tomography solution
KEYNOTE 16:10-17:10 Remote	Kristina Micheva, Senior Research Scientist at Stanford University School of Medicine, California, United States. She is a neuroscientist at Stanford University and one of the main developers of Array Tomography technique. Title: Correlative AT of synapses: How light and electron microscopy approaches benefit from each other
17:10-17:40	Group discussions
18:00	Welcome Reception

November 30th

Time	Event
Session 5	New frontiers – Array Tomography from sample preparation to image processing & analysis
9:00-9:30	José Maria Mateos Melero, Project Manager, Center for Microscopy and Image Analysis, University of Zurich, Switzerland Title: From samples to Results in an Imaging Facility: Optimized Array Tomography Workflows for Users-Driven Project
9:30-10:00	Norman Rzepka, Managing Director at Scalable Minds GmbH Title: Data analysis, management and sharing for large-scale volume EM with WEBKNOSSOS
KEYNOTE 10:00-11:00	Moritz Helmstaedter, Max Planck Institute for Brain Research, Germany Title: Measuring communication maps of neuronal circuits, connectomes
11:00-11:30	Coffee-break
11:30-11:50	ZEISS, Joerg Lindenau, Company Presentation Title: Fast and precise preparation of resin embedded brain tissue by Femtosecond Ablation Laser for targeted Volume EM
11:50-12:10	RMC Boeckeler, Gareth Morgan, Company Presentation Title: ATUM: Large and Small Scale Section Collection
12:10-12:40 Remote	Thomas Templier, Research Specialist, Janelia Research Campus, United States Title: IBEAM-MSEM
12:40-12:50	Closing remarks
12:50	Grab and go lunch

Program - Hands-on

Venue: Centre for Cellular Imaging

Workshops:

RMC Boeckeler, Gareth Morgan - Sample preparation: Serial section collection by Automated Tape Ultramicrotome
LEICA, Jan de Weert - Sample preparation: Leica ARTOS 3D Array Tomography Solution Get Quality Serial Sections
CARL ZEISS, Ralph Neujahr - High Resolution TEM-like imaging with your SEM: Serial Sections and Array Tomography
THERMO FISHER, Sarah Neumann - Maps software for resin section imaging and correlation
Richard Schalek, Harvard University - Array Tomography processing and analysis: Neuroglancer and VAST, to explore and mine publically shared EM datasets
Scalableminds, Norman Rzepka - Collaborative annotation, data management and sharing for large-scale 3D images with WEBKNOSSOS

November 30th

Time	Event
13:30-15:00	RMC Boeckeler Gareth Morgan LEICA Jan de Weert	CARL ZEISS Ralph Neujahr	THERMO FISHER Sarah Neumann	Harvard University Richard Schalek	Scalableminds Norman Rzepka
15:00-15:30	Coffee-break
15:30-17:00	RMC Boeckeler Gareth Morgan LEICA Jan de Weert	CARL ZEISS Ralph Neujahr	THERMO FISHER Sarah Neumann	Harvard University Richard Schalek	Scalableminds Norman Rzepka

Time

Event

13:30-15:00

RMC Boeckeler

Gareth Morgan

LEICA

Jan de Weert

CARL ZEISS

Ralph Neujahr

THERMO FISHER

Sarah Neumann

Harvard University

Richard Schalek

Scalableminds

Norman Rzepka

15:00-15:30

Coffee-break

15:30-17:00