MPE provides research support within recombinant protein production and mammalian cell culture. We also perform Western blots and some other protein analyses.
Production of Recombinant Proteins
We produce recombinant proteins in mammalian cells, which gives the possibility to produce complex proteins with post-translational modifications, such as glycosylation and disulfide bonds, and also proteins consisiting of several subunits. Mammalian cells are also good for larger, secretory proteins, which can be difficult to express in lower species.
The production of recombinant proteins in mammalian cells involves several steps (expression vector, production and purification of the protein), that we can perform at the centre. We can also give advice on the different steps. If you want to express a protein with us, please contact us for a discussion on an appropriate approach for your needs.
The MPE facility has equipment for the small and large scale culture of most mammalian cells, growing either attached or in suspension. This means that we have the ability to culture many different types of cell lines. If you have an interest in having a cell line cultured at MPE, please contact us.
We perform SDS-PAGE and Western blot for analysis of produced proteins. We can also analyze the presence of glycans on the protein. Analytical size-exclusion chromatography (SEC) can be used for verification of protein size and form.
We use Tangential Flow Filtration (TFF) for concentration and buffer exchange of produced proteins in volumes up to 15 litres.
Purification of the protein can be performed by different chromatographic techniques such as immobilized metal affinity chromatography (IMAC), anion exchange or gel filtration (SEC).
Test for mycoplasma
Test for mycoplasma-infection of cell lines. This is mandatory when cell lines are brought into MPE, but we can also test other cells.
Below you can find a list of equipment available at MPE. If you have any questions, please contact us.
Large cell culture laboratory with several LAF benches
Incubators for adherent cell cultivation in plates T-flasks
Several Shake Incubators
For cultivation and transfection of suspension cells in E-flasks up to 2L in different temperatures.
Incubator with magnetic stirrer for spinner flask cultivation
Two bioreactors (bench top) for large-scale suspension cultivation (batch or perfusion mode)
Cell Counter, CytoSmart
Tangential Flow Filtration
Equipment for concentration of product
Electrophoresis equipment for product analysis
ÄKTA pure with different separation columns för purification and analysis (Anion, SEC, IMAC).
Cryofreezer (-152°C) for long term storage
For measurement of the concentration of proteins and DNA
For larger scale preparation of DNA (for transient transfection)