The Proteomics Core Facility is a research infrastructure available for researchers at Sahlgrenska Academy at Gothenburg University and external customers from other universities or industry.
Our research support includes study design, protein identification, characterization of post-translational modifications, as well as protein interaction analysis. The majority of projects we are involved in are discovery proteomic analyses, in which thousands of proteins can be identified and quantified in an unbiased manner.
The Proteomics Core Facility has experience regarding preparation of a huge range of samples originating from tissues, cells, isolated cellular compartments, pull-downs, other biological samples, or expressed proteins. High pH liquid chromatography fractionation prior to nano LC-MS is used for in-depth analysis and to study lower abundant proteins in complex biological samples. The facility is equipped with the latest high-resolution mass spectrometers.
We provide comprehensive data analysis through a variety of database search engines (Mascot, Sequest) and software packages (Proteome Discoverer, Byonics and MaxQuant). PCF is involved in approximately 300 projects per year.