The Proteomics Core Facility (PCF) is a research infrastructure available to the researchers at Sahlgrenska Academy at Gothenburg University and Chalmers as well as external customers from other universities or industry. We are involved in approximately 300 projects per year.
The facility is equipped with high-resolution mass spectrometers. We offer different research support including study design, protein identification, characterization of post-translational modifications e.g. phosphorylation and glycosylation, and protein-protein interaction analysis. Global quantitative analyses of differentially expressed proteins are available using Tandem Mass Tag (TMT).
The Proteomics Core Facility has experience of sample preparation of a range of species and material such as tissues, cells, isolated cellular compartments, pull-downs, other biological samples, or expressed proteins. High pH liquid chromatography fractionation prior to nLCMS is used for in-depth analysis and to study lower abundant proteins in complex biological samples.
We provide comprehensive data analysis through a variety of database search engines (Mascot, Sequest) and software packages Proteome Discoverer, Byonics and MaxQuant.