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AxioImager

The upright AxioImager.Z2 from Carl Zeiss is a higly automatic widefield microscope, which is controlled by the Zen Blue software.

With this microscope it is possible to perform multichannel images, z-stacks, time series and, perhaps most important, to aquire large tile overviews using stitching. 

The microscope is equipped with dual cameras:

  • A 14 bit high-performance color camera, Axiocam 506 color, with a frame size of 2752 × 2208 pixels, suitable for histologically stained specimens
  • A sensitive 14 bit black/white camera, Axiocam 506 mono, , with a frame size of 2752 × 2208 pixels, for fluorescence imaging. 

Excitation wavelengths

The lamp unit Colibri 7 has the following excitation wavelenths:
385 nm, 430 nm, 475 nm, 555 nm, 590 nm, 630 nm and 735 nm

Fluorescence filters

There are six filter sets, three single-band and three multi-band:


Filter set 96 HE BFP    

Emission filter BP 450/40, Colibri excitation 385 nm

Filter set 38 HE GFP 

Emission filter BP 525/50, Colibri excitation 475 nm

Filter set 43 HE  DsRed  

Emission filter BP 605/70, Colibri excitation 555 nm

Filter set 90 HE DAPI/GFP/Cy3/Cy5   

Emission filter QBP 425/30+514/30+592/25+709/100, Colibri excitation 385, 475, 555 and/or 631 nm

Filter set 91 HE  CFP/YFP/mCherry  

Emission filter TBP 467/24+555/25+687/145, Colibri excitation 423, 511 and/or 591 nm

Filter set 110 HE  DAPI/GFP/Cy3.5/Cy7

Emission filter QBP 410-440+501-547 + 618-650 + 770-800, Colibri excitation 385, 475, 590 and/or 735 nm

Objectives

The objectives in the list below are installed on the microscope. For other needs, please ask the CCI staff.

  • EC Plan-Neufluar 5x/0.16 Ph 1
  • EC Plan-Neufluar 10x/0.30 Ph 1
  • Plan-Apochromat 20x/0.8 
  • C-Apochromat 40x/1.20 W Korr
  • EC Epiplan 50x/0.75 H