From Image Acquisition to Image Analysis

Master’s level
5 credits (ECTS)


This is a compact, hands-on from image acquisition to image analysis course. The example material used is mainly fluorescent bio-images, but the underlying concepts taught apply also to images from electron microscopy.


The focus of the course is on reaching a broad understanding of imaging acquisition and computerized image analysis in Life Science and a basic understanding of the theory and algorithms behind the image analysis methods and tools. Hands-on exercises that will teach a broad functionality of these tools and the concepts needed for building suitable workflow for a given application.

This course provides:

  • basic concepts in modern fluorescence microscopy: What is fluorescence, different fluorochromes, excitation methods, emission characteristics, purpose and uses
  • basic concepts of digital images;
  • basic computerized image analysis methods and computer exercises;
    image processing in preparation for analysis;
  • noise and image enhancement; and
  • automatically analyzing large datasets.

    The course will also:

  • address the issues of image ethics and proper image acquisition for image analysis; and
  • offer an introduction to a number of freely available software tools (Image/Fiji).

The course will be conducted in the lecture-hall, the Centre for Cellular Imaging facility, and in the computer classroom, one user for each computer. It will consist of both theoretical and practical classes with demos and hands-on at the microscope and analysis of image examples.

The course will be given in English.

Prerequisites and selection


For admission to the course, the Bachelor's degree is required 180 credits or equivalent in any of the fields of medicine, nursing or natural sciences and English 6.