Using Single cell RNA-sequencing for understanding the perinatal brain
Single cell RNA-sequencing is a recently developed technique that allows studying the biological function of different types of cells, one cell at a time. This method is a focus area at the Bioinformatics Core Facilities. Bioinformatician Vanja Börjesson has recently been involved in supporting a research project that resulted in new findings on the field of perinatal brain injury, showing that the neonatal meninges contain almost all known types of immune cells.
Single cell RNA-sequencing, or snRNA-seq technique in short, is useful to use when you are interested in e.g.,
1) knowing what subtypes of cells you have in your sample/tissue,
2) finding differences on a transcriptional (RNA) level between samples within one subpopulation,
3) knowing how cells in a sample or subpopulations of cells in a sample develop/diverge over time, or
4) finding rare cell populations.