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Noncoding small RNAs in health and disease
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Noncoding small RNAs in health and disease

Research group
Active research
Project owner
Institute of Biomedicine

Short description

miRNAs are small non-coding RNA molecules that are expressed in a cell type-specific manner and can accumulate to thousands of copies per cell. Together with the Argonaute (AGO) protein, the miRNA silence other RNA molecules by binding complementary sequences within them. This regulation takes place within the cytoplasm of the cell. AGO miRNAs also contribute to gene regulation in the nucleus of the cell and we are investigating the mechanisms of this regulation.

Argonaute (AGO) proteins, loaded with small interfering RNAs (siRNAs) or microRNAs (miRNAs), are the core components of RNA induced silencing complex (RISC). The small RNAs guide the AGO ribonucleoprotein to complementary target RNAs resulting in gene silencing. microRNAs (miRNAs), 21-23 nucleotide long non-coding RNA molecules, are expressed in a cell type-specific manner and can accumulate to tens of thousands of copies per cell. It is estimated that up to 60% of human genes are under miRNA regulation. miRNA silencing pathways play important roles in a number of biological processes, including embryonic stem cell differentiation and tissue development. Deregulation of this pathway is associated with various diseases; therefore, it is essential to fully understand the molecular mechanisms of miRNA regulation.    

We recently discovered that key factors of the RISC silencing complex are expressed in the nucleus of embryonic stem cells. Our current work aims at decoding the regulatory pathways of nuclear RISC. Specifically, we want to understand why stem cells need nuclear miRNA-Argonaute complexes for gene regulation and how these play a role during stem cell differentiation and reprograming. We further aim to understand if nuclear RNA interference by RISC can be used in cancers as a way of targeting nuclear factors important in cancer development.    

  • New insight into role of myosin motors for activation of RNA polymerases.

    Authors: Aishe A. Sarshad, Piergiorgio Percipalle

    Publication type:Review article, reviewed

    Publication year:2014

    Published in:International review of cell and molecular biology
     

  • PAR-CLIP: A Genomic Technique to Dissect RNA-Protein Interactions

    Authors: Tara Dutka, Aishe A. Sarshad, Markus Hafner

    Publication type:Book chapter, reviewed

    Publication year:2016

    Published in:Aransay A., Lavín Trueba J. (eds) Field Guidelines for Genetic Experimental Designs in High-Throughput Sequencing.
     

  • PAR-CLIP and streamlined small RNA cDNA library preparation protocol for the identification of RNA binding protein target sites.

    Authors: Daniel Benhalevy, Hannah L McFarland, Aishe A. Sarshad, Markus Hafner

    Publication type:Journal article, reviewed

    Publication year:2017

    Published in:Methods (San Diego, Calif.)

  • Nuclear myosin 1 contributes to a chromatin landscape compatible with RNA polymerase II transcription activation.

     

    Authors: Bader Almuzzaini, Aishe A. Sarshad, Ann-Kristin Östlund Farrants, Piergiorgio Percipalle

    Publication type:Journal article, reviewed

    Publication year:2015

    Published in:BMC biology
     

  • Identification, Location, and Emergence of the Cercaria of Leuceruthrus micropteri (Trematoda: Azygiidae) Recovered from the Snail Pleurocera semicarinata (Gastropoda: Pleuroceridae) at North Elkhorn Creek, Kentucky

    Authors: R. Rosen, C. Peng, B. Traw, E. Blevins, B. Jovanovic, Aishe A. Sarshad, F. Zaki

    Publication type:Journal article, reviewed

    Publication year:2019

    Published in:Comparative Parasitology
     

  • Glycogen synthase kinase (GSK) 3β phosphorylates and protects nuclear myosin 1c from proteasome-mediated degradation to activate rDNA transcription in early G1 cells.

    Authors: Aishe A. Sarshad, Martin Corcoran, Bader Al-Muzzaini, Laura Borgonovo-Brandter, Anne Von Euler, Douglas Lamont, Neus Visa, Piergiorgio Percipalle

    Publication type:Journal article, reviewed

    Publication year:2014

    Published in:PLoS genetics
     

  • 6mer seed toxicity in tumor suppressive microRNAs.

    Authors: Quan Q Gao, William E Putzbach, Andrea E Murmann, Siquan Chen, Aishe A. Sarshad, Johannes M Peter, Elizabeth T Bartom, Markus Hafner, Marcus E Peter

    Publication type:Journal article, reviewed

    Publication year:2018

    Published in:Nature communications
     

  • Nuclear myosin 1c facilitates the chromatin modifications required to activate rRNA gene transcription and cell cycle progression.

    Authors: Aishe A. Sarshad, Fatemeh Sadeghifar, Emilie Louvet, Raffaele Mori, Stefanie Böhm, Bader Al-Muzzaini, Anna Vintermist, Nathalie Fomproix, Ann-Kristin Östlund, Piergiorgio Percipalle

    Publication type:Journal article, reviewed

    Publication year:2013

    Published in:PLoS genetics
     

  • CD95/Fas ligand mRNA is toxic to cells.

    Authors: Will Putzbach, Ashley Haluck-Kangas, Quan Q Gao, Aishe A. Sarshad, Elizabeth T Bartom, Austin Stults, Abdul S Qadir, Markus Hafner, Marcus E Peter

    Publication type:Journal article, reviewed

    Publication year:2018

    Published in:eLife
     

  • In β-actin knockouts, epigenetic reprogramming and rDNA transcription inactivation lead to growth and proliferation defects.

    Authors: Bader Almuzzaini, Aishe A. Sarshad, Aldwin S Rahmanto, Magnus L Hansson, Anne Von Euler, Olle Sangfelt, Neus Visa, Ann-Kristin Östlund Farrants, Piergiorgio Percipalle

    Publication type:Journal article, reviewed

    Publication year:2016

    Published in:FASEB journal : official publication of the Federation of American Societies for Experimental Biology
     

  • Argonaute-miRNA Complexes Silence Target mRNAs in the Nucleus of Mammalian Stem Cells.

    Authors: Aishe A. Sarshad, Aster H Juan, Ana Iris Correa Muler, Dimitrios G Anastasakis, Xiantao Wang, Pavol Genzor, Xuesong Feng, Pei-Fang Tsai, Hong-Wei Sun, Astrid D Haase et al.

    Publication type:Journal article, reviewed

    Publication year:2018

    Published in:Molecular cell
     

  • Many si/shRNAs can kill cancer cells by targeting multiple survival genes through an off-target mechanism.

    Authors: William Putzbach, Quan Q Gao, Monal Patel, Stijn van Dongen, Ashley Haluck-Kangas, Aishe A. Sarshad, Elizabeth T Bartom, Kwang-Youn A Kim, Denise M Scholtens, Markus Hafner et al.

    Publication type:Journal article, reviewed

    Publication year:2017

    Published in:eLife
     

  • Wnt5a Signals through DVL1 to Repress Ribosomal DNA Transcription by RNA Polymerase I.

    Authors: Randall A Dass, Aishe A. Sarshad, Brittany B Carson, Jennifer M Feenstra, Amanpreet Kaur, Ales Obrdlik, Matthew M Parks, Varsha Prakash, Damon K Love, Kristian Pietras et al.

    Publication type:Journal article, reviewed

    Publication year:2016

    Published in:PLoS genetics
     

  • miR-450a Acts as a Tumor Suppressor in Ovarian Cancer by Regulating Energy Metabolism.

    Authors: Bruna Rodrigues Muys, Josane F Sousa, Jessica Rodrigues Plaça, Luíza Ferreira de Araújo, Aishe A Sarshad, Dimitrios G Anastasakis, Xiantao Wang, Xiao Ling Li, Greice Andreotti de Molfetta, Anelisa Ramão et al.

    Publication type:Journal article, reviewed

    Publication year:2019

    Published in:Cancer research
     

  • A Muscle-Specific Enhancer RNA Mediates Cohesin Recruitment and Regulates Transcription In trans.

    Authors: Pei-Fang Tsai, Stefania Dell'Orso, Joseph Rodriguez, Karinna O Vivanco, Kyung-Dae Ko, Kan Jiang, Aster H Juan, Aishe A. Sarshad, Laura Vian, Michelle Tran et al.

    Publication type:Journal article, reviewed

    Publication year:2018

    Published in:Molecular cell

Aishe Sarshad, group leader

Group members

Aishe Sarshad

Vivian Lobo