
Noncoding small RNAs in health and disease
Short description
miRNAs are small non-coding RNA molecules that are expressed in a cell type-specific manner and can accumulate to thousands of copies per cell. Together with the Argonaute (AGO) protein, the miRNA silence other RNA molecules by binding complementary sequences within them. This regulation takes place within the cytoplasm of the cell. AGO miRNAs also contribute to gene regulation in the nucleus of the cell and we are investigating the mechanisms of this regulation.
Argonaute (AGO) proteins, loaded with small interfering RNAs (siRNAs) or microRNAs (miRNAs), are the core components of RNA induced silencing complex (RISC). The small RNAs guide the AGO ribonucleoprotein to complementary target RNAs resulting in gene silencing. microRNAs (miRNAs), 21-23 nucleotide long non-coding RNA molecules, are expressed in a cell type-specific manner and can accumulate to tens of thousands of copies per cell. It is estimated that up to 60% of human genes are under miRNA regulation. miRNA silencing pathways play important roles in a number of biological processes, including embryonic stem cell differentiation and tissue development. Deregulation of this pathway is associated with various diseases; therefore, it is essential to fully understand the molecular mechanisms of miRNA regulation.
We recently discovered that key factors of the RISC silencing complex are expressed in the nucleus of embryonic stem cells. Our current work aims at decoding the regulatory pathways of nuclear RISC. Specifically, we want to understand why stem cells need nuclear miRNA-Argonaute complexes for gene regulation and how these play a role during stem cell differentiation and reprograming. We further aim to understand if nuclear RNA interference by RISC can be used in cancers as a way of targeting nuclear factors important in cancer development.
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New insight into role of myosin motors for activation of RNA polymerases.
Authors: Aishe A. Sarshad, Piergiorgio Percipalle
Publication type:Review article, reviewed
Publication year:2014
Published in:International review of cell and molecular biology
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PAR-CLIP: A Genomic Technique to Dissect RNA-Protein Interactions
Authors: Tara Dutka, Aishe A. Sarshad, Markus Hafner
Publication type:Book chapter, reviewed
Publication year:2016
Published in:Aransay A., Lavín Trueba J. (eds) Field Guidelines for Genetic Experimental Designs in High-Throughput Sequencing.
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PAR-CLIP and streamlined small RNA cDNA library preparation protocol for the identification of RNA binding protein target sites.
Authors: Daniel Benhalevy, Hannah L McFarland, Aishe A. Sarshad, Markus Hafner
Publication type:Journal article, reviewed
Publication year:2017
Published in:Methods (San Diego, Calif.)
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Nuclear myosin 1 contributes to a chromatin landscape compatible with RNA polymerase II transcription activation.
Authors: Bader Almuzzaini, Aishe A. Sarshad, Ann-Kristin Östlund Farrants, Piergiorgio Percipalle
Publication type:Journal article, reviewed
Publication year:2015
Published in:BMC biology
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Identification, Location, and Emergence of the Cercaria of Leuceruthrus micropteri (Trematoda: Azygiidae) Recovered from the Snail Pleurocera semicarinata (Gastropoda: Pleuroceridae) at North Elkhorn Creek, Kentucky
Authors: R. Rosen, C. Peng, B. Traw, E. Blevins, B. Jovanovic, Aishe A. Sarshad, F. Zaki
Publication type:Journal article, reviewed
Publication year:2019
Published in:Comparative Parasitology
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Glycogen synthase kinase (GSK) 3β phosphorylates and protects nuclear myosin 1c from proteasome-mediated degradation to activate rDNA transcription in early G1 cells.
Authors: Aishe A. Sarshad, Martin Corcoran, Bader Al-Muzzaini, Laura Borgonovo-Brandter, Anne Von Euler, Douglas Lamont, Neus Visa, Piergiorgio Percipalle
Publication type:Journal article, reviewed
Publication year:2014
Published in:PLoS genetics
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6mer seed toxicity in tumor suppressive microRNAs.
Authors: Quan Q Gao, William E Putzbach, Andrea E Murmann, Siquan Chen, Aishe A. Sarshad, Johannes M Peter, Elizabeth T Bartom, Markus Hafner, Marcus E Peter
Publication type:Journal article, reviewed
Publication year:2018
Published in:Nature communications
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Nuclear myosin 1c facilitates the chromatin modifications required to activate rRNA gene transcription and cell cycle progression.
Authors: Aishe A. Sarshad, Fatemeh Sadeghifar, Emilie Louvet, Raffaele Mori, Stefanie Böhm, Bader Al-Muzzaini, Anna Vintermist, Nathalie Fomproix, Ann-Kristin Östlund, Piergiorgio Percipalle
Publication type:Journal article, reviewed
Publication year:2013
Published in:PLoS genetics
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CD95/Fas ligand mRNA is toxic to cells.
Authors: Will Putzbach, Ashley Haluck-Kangas, Quan Q Gao, Aishe A. Sarshad, Elizabeth T Bartom, Austin Stults, Abdul S Qadir, Markus Hafner, Marcus E Peter
Publication type:Journal article, reviewed
Publication year:2018
Published in:eLife
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In β-actin knockouts, epigenetic reprogramming and rDNA transcription inactivation lead to growth and proliferation defects.
Authors: Bader Almuzzaini, Aishe A. Sarshad, Aldwin S Rahmanto, Magnus L Hansson, Anne Von Euler, Olle Sangfelt, Neus Visa, Ann-Kristin Östlund Farrants, Piergiorgio Percipalle
Publication type:Journal article, reviewed
Publication year:2016
Published in:FASEB journal : official publication of the Federation of American Societies for Experimental Biology
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Argonaute-miRNA Complexes Silence Target mRNAs in the Nucleus of Mammalian Stem Cells.
Authors: Aishe A. Sarshad, Aster H Juan, Ana Iris Correa Muler, Dimitrios G Anastasakis, Xiantao Wang, Pavol Genzor, Xuesong Feng, Pei-Fang Tsai, Hong-Wei Sun, Astrid D Haase et al.
Publication type:Journal article, reviewed
Publication year:2018
Published in:Molecular cell
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Many si/shRNAs can kill cancer cells by targeting multiple survival genes through an off-target mechanism.
Authors: William Putzbach, Quan Q Gao, Monal Patel, Stijn van Dongen, Ashley Haluck-Kangas, Aishe A. Sarshad, Elizabeth T Bartom, Kwang-Youn A Kim, Denise M Scholtens, Markus Hafner et al.
Publication type:Journal article, reviewed
Publication year:2017
Published in:eLife
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Wnt5a Signals through DVL1 to Repress Ribosomal DNA Transcription by RNA Polymerase I.
Authors: Randall A Dass, Aishe A. Sarshad, Brittany B Carson, Jennifer M Feenstra, Amanpreet Kaur, Ales Obrdlik, Matthew M Parks, Varsha Prakash, Damon K Love, Kristian Pietras et al.
Publication type:Journal article, reviewed
Publication year:2016
Published in:PLoS genetics
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miR-450a Acts as a Tumor Suppressor in Ovarian Cancer by Regulating Energy Metabolism.
Authors: Bruna Rodrigues Muys, Josane F Sousa, Jessica Rodrigues Plaça, Luíza Ferreira de Araújo, Aishe A Sarshad, Dimitrios G Anastasakis, Xiantao Wang, Xiao Ling Li, Greice Andreotti de Molfetta, Anelisa Ramão et al.
Publication type:Journal article, reviewed
Publication year:2019
Published in:Cancer research
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A Muscle-Specific Enhancer RNA Mediates Cohesin Recruitment and Regulates Transcription In trans.
Authors: Pei-Fang Tsai, Stefania Dell'Orso, Joseph Rodriguez, Karinna O Vivanco, Kyung-Dae Ko, Kan Jiang, Aster H Juan, Aishe A. Sarshad, Laura Vian, Michelle Tran et al.
Publication type:Journal article, reviewed
Publication year:2018
Published in:Molecular cell

Aishe Sarshad
Principal Investigator
Affiliation:
Department of Medical Biochemistry and Cell Biology,
Institute of Biomedicine
Group members
Aishe Sarshad
Vivian Lobo