Liver-on-chip for drug discovery and personalized medicine, 2018-
(SSF Works; Instrument, Technique, and Method Development Projects 2017)
An interdisciplinary research project in which a realistic model of the human liver tissue will be monitored in real-time with light-sheet microscopy and validated by studying liver metabolism and disease, in particular NAFLD, fibrosis and liver cancer.
Senior lecturer Giovanni Volpe, Department of Physics, GU
Prof. Stefano Romeo, Wallenberg Laboratory, SA, GU
Assistant Prof. Daniel Midtvedt, Department of Physics, GU
Individual Glycolytic Oscillations to Propagating Waves
Delineating the metabolic pathway of glycolysis present in eukaryotic cells. So far, our focus has been to investigate the individual cellular glycolytic oscillations, their entrainment of each other to reach a synchronized bulk behaviour and the network regulation. The single-cell data are vital to understanding the transitions between “out-of-phase” to “in-phase” oscillations. Experimental data complemented with numerical analysis and systems biology.
Prof. Jacky L. Snoep, Stellenbosch University, South Africa
Prof. Bernhard Mehlig, Department of Physics, GU
Senior Lecturer Giovanni Volpe, Department of Physics, GU
ITN funded by the European Union’s Horizon 2020 research and innovation program under grant agreement No. 766181
The liver contains a large number of very fine capillaries (the sinusoids), which are lined by endothelial cells. In the liver, these endothelial cells are called liver sinusoidal endothelial cells (LSEC) and they contain thousands of nanosized pores (fenestrations) that enable the clearance of molecules and small particles from the blood. The size of these fenestrations is well below the optical diffraction limit, and consequently, very little is known about the essential physiological function of these unique structures and their role in the transfer and/or clearance of metabolites and pharmaceuticals to vital organs. LSECs are also responsible for the removal and degradation of pharmaceuticals, virus and waste macromolecules, making them a highly relevant type of cell for the study of pharmacological drug uptake. Because of the issues mentioned above, however, most pharmaceutical companies currently cannot assess the effect of drugs on these cells. Optical nanoscopy techniques and microfluidic chip fabrication aid the studies and characterization of the fenestrations in different microenvironmental conditions.
Collaborators: Consortia members distributed at 6 universities and 3 SMEs across Europe with additional partners from across the world.
ITN funded by the European Union’s Horizon 2020 research and innovation program under grant agreement No. 812780.
The project focuses on experimental, theoretical and computational aspects of active matter. The specific aim of the ACTIVEMATTER network is to “prepare a new generation of physicists and engineers with the scientific insight and managerial skills to harness active matter at mesoscopic and nanoscopic length- scales and to exploit it in high-impact applications (e.g. the design and fabrication of biomimetic materials, the targeted localization, pick-up and transport of nanoscopic cargoes in drug delivery, bioremediation and chemical sensing)”.
Collaborator: One external PhD student at the SME Elvesys and consortia members from 9 different countries.