Single Molecule Localization Microscopy
Single Molecule Localization Microscopy (SMLM) is the common name of several techniques, which utilizes the blinking of fluorophores to surpass the diffraction limit, such as PALM, dSTORM etc. In all of these techniques, a small subset of fluorophores are imaged in each frame of a long time series. The localization of each detected flourophore is found and the final superresolution image is reconstructed from all localization positions.
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The principle of SMLM: Instead of collecting the signal from all the labeled molecules in the sample at the same time, like in the widefield (WF) technique, only a few molecules are imaged in each frame of a time series.