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University of Gothenburg
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PALM MicroBeam

Laser microdissection and pressure catapulting microscope from Carl Zeiss

The PALM MicroBeam microscope from Carl Zeiss is a Laser Microdissection and Pressure Catapulting (LMPC) system. 

It was purchased using a grant from the Swedish Research Council and the main applicant, Prof. Marianne Quiding-Järbrink at the Dept of Microbiology and Immunology, kindly placed it at the CCI in 2008.

Microscope

This laser microdissection system is based on a fully motorized inverted microscope, the AxioObserver Z1, equipped with a RoboMover collector control unit, a pulsed 355 nm laser for cutting and two different cameras: an AxioCam ICc1, a color camera for bright field imaging, and an AxioCam MRm, a black and white camera for fluorescence imaging. The microscope is controlled by the PALM RoboSoftware. 

Fluorecsence filters

For fluorescence imaging there is a Colibri illumination module, with light emitting diodes (LEDs) giving three different excitation wavelengths: 365 nm, 470 nm and 585 nm, and a triple filter (Filter set 62 HE BFP GFP HcRed shift free) for fluorescence imaging. The triple filter has the emission bandwidths 402 – 448 nm, 500 - 554 nm, and > 615 nm for blue, green and red fluorescence.

Objectives

  • Fluar 5x/0.25 
  • Fluar 10x/0.50
  • LD Plan-Neofluar 20x/0.40 Corr 
  • LD Plan-Neofluar 40x/0.60 Corr 
  • LD "Plan-Neofluar" 63x/0.75 Corr
  • Fluar 100x/1.30 Oil UV 
     

Sample holders 

There are five different sample holders for:

  • up to three regular 1 mm glass slides
  • a 50 mm 1 mm glass slide
  • up to three 0.17 mm cover glass slides
  • a 35 mm cell culture dish
  • a 50 mm cell culture dish
     

Collectors 

There are five different sample holders for:

  • a single 500 µl tube, with a diffuser, to be used with glass slides 
  • a single 500 µl tube, without a diffuser, to be used with cell culture dishes
  • up to eight 500 µl tubes
  • up to eight 200 µl tubes
  • up to twelve 8-cap strips
  • a CapturePlate 96