LSM980 with Airyscan 2
The Carl Zeiss LSM 980 with Airyscan 2 is a state-of-the-art inverted microscope designed for imaging in both laser scanning confocal and the novel super-resolution technique Airyscan. The system is dedicated to fast and sensitive live cell imaging with a full incubation chamber. Further, the LSM980 is optimized for simultaneous spectral detection of multiple weak labels with the highest light efficiency.
LSM 980
The LSM980 is a confocal microscope optimized for simultaneous detection of multiple weak labels. New multiplexing modalities allow for fast volumetric imaging, live experiments, spectral imaging, and more.
Airyscan 2
Airyscan is a method for gentle super-resolution imaging based on the Line scanning SIM by Heintzmann and the use of a imaging sensor as demonstrated by Müller and Enderlein. In Airyscan imaging the sample is scanned as in confocal, while the emitted light is captured by a sensor. Further, new multiplexing modalities allow to capture larger fields of view or dynamic processes using shorter acquisition times than previously possible.
Enhanced Software Tools for Productivity
The ZEN software has available several modules that allow to define complex tasks, and achieve reproducible results. The AI Sample Finder helps to quickly define regions of interest in large samples. Smart Setup is able to suggest imaging settings based on the modality of interest and labels present on the sample. Direct Processing enables parallel acquisition and data processing. ZEN Connect can be used to handle complex multimodal and correlative imaging approaches, making it easy to overlay and organize images from any source.
Imaging modalities of the LSM 980 with Airyscan 2
Built for navigating and correlating with ease in complex samples. Multicolour images can be acquired with the Widefield system and then used to manual or automated navigation into the sample for more detail confocal studies. This workflow is also improved by the use of the ZEN connect and Guided Acquisition software.
A light-efficient beam path with up to 34 simultaneous channels and full spectral flexibility results in a great confocal for multi-color experiments with living samples. The unique combination of spectral imaging with improved signal-to-noise ratio and resolution enables lower laser power for your live cell experiments.
To separate even highly overlapping signals or to remove autofluorescence, the Lambda Scan can be used to measure up to 32 distinct spectral channel with an Spectral resolution of 8.9 nm. Then spectral unmixing can be used to recover the occurrence of each fluorophore. This can be done using ZEN blue or home built algorithms developed at CCI.
Airyscan 2 is an area detector with 32 honeycomb arranged detection elements. Due to their physical size and the use of a telescope in the beam path, each element acts as a pinhole, contributing to super-resolution information, while the complete detector area collects more light than the standard confocal setting.
In Multiplex modes, Airyscan detector advantages are combined with adapted illumination and readout schemes, giving different choices for parallelization (faster imaging speeds).
Multiplex modes of the LSM980
| Airyscan | SR | SR-4Y | SR-8Y | CO-8Y |
|---|---|---|---|---|
| Parallelization | 1 | 4 | 8 | 8 |
| Resolution [nm] | 120/120 | 140/140 | 120/160 | Confocal |
| Max FPS | 0.2 (Zoom 1.7) | 1.0 (Zoom 1) | 2.0 (Zoom 1) | 9.6 (Zoom 1) |
| Fine structures | +++++ | ++++ | +++ | ++ |
| Tiling | ++ | ++++ | ++++ | +++ |
| Live cell imaging | ++ | +++ | ++++ | +++++ |
LSM980 system characteristics
Objective lenses
Air objectives
- EC Plan-Neofluar 10X / 0.3 external link
- Plan-Apochromat 20X / 0.8 external link
Immersion objectives
- Plan-Apochromat 63X / 1.4 Oil external link
Widefield module
WF Transmission
- Halogen Lamp
WF Fluorescence LEDs Colibri
- 385 nm
- 475 nm
- 555 nm
- 630 nm
WF Dichroic filter
QUAD filterset:
Excitation: 385 nm (370-400) 475 nm (450-488) 555 nm (540-570) & 630 nm (614-647)
Emission: BP 410-440 nm, BP 499-529 nm, BP 579-604 nm & BP 659-759 nm
Camera: Axiocam 705 mono, 5 megapixel monochrome CMOS camera, 3.45 μm pixel size. 60 frames per second with full 5 megapixels or faster after cropping. Peak Quantum efficiency of up to 72 % and a broad spectral sensitivity ranging from UV to near-IR light.
Confocal module
Lasers
- 405 nm
- 488 nm
- 561 nm
- 639 nm
2 x PMT Multialkaline Detectors
32-channel GaAsp Detector
Airyscan 2 module
Airyscan 2 GaAsP detector: is an area detector with 32 honeycomb arranged detection elements. Due to their physical size and the use of a telescope in the beam path, each element acts as a pinhole, contributing to super-resolution information, while the complete detector area collects more light than the standard confocal setting.
- BP 420-480 nm + BP 495-550 nm
- BP 420-480 nm + BP 570-630 nm
- BP 420-500 nm + LP 605 nm
- BP 465-505 nm + BP 525-585 nm
- BP 495-550 nm + BP 570-630 nm
- BP 495-555 nm + LP 660 nm
- BP 570-620 nm + LP 660 nm
