Specimen Preparation Techniques for EM
Visualizing a biological specimen with an electron microscope is not a trivial task, mostly because of the intrinsic nature of the electron and matter interactions that are responsible for the image formation. In order to be imaged using TEM the specimen has to be very thin and for using SEM the specimen needs a conductive surface. In both cases the specimen has to be placed inside high vacuum. Thus, biological specimens cannot be imaged in their native state and need to be heavily processed. Accordingly, sample preparation is a crucial step, which represents an essential activity and service in our facility.