EATRIS Center for Large-scale cell-based screening

1. High-content, high throughput, imaging based screening (HCS)
2. High resolution, high-throughput quantitative growth phenotyping (liquid and solid medium growth phenotyping)
3. Synthetic gene arrays: genome-wide genetic interaction screens
4. Chemical genetics: bioactive molecules
5. Experimental evolution
6. High-content image-based mammalian cell screening service.

Written applications (max. 3 A4 pages) should be sent to the Director of Infrastructure Beidong Liu, beidong.liu@cmb.gu.se. If necessary, the Director may request a meeting with potential users to discuss the project in detail. And the project will be evaluated based on the criteria listed below. The results of the decision will be announced within 4 weeks after submission.

Criteria to be evaluated
1. Feasibility of the project
2. Scientific quality
3. Innovation potential
4. Availability of equipment in the light of competing project

User groups and priority levels
1. Academic users
1. 1. External academic users, whether within the department, faculty or the University of Gothenburg.
A fee will be applied according to any of the service models below.

1.2. Internal academic users for course teaching.
A fee can be charged to the teaching funds of the department, if approval is obtained from the director of undergraduate teaching.

1.3. Infrastructure running and maintenance groups. These are the research groups from the research environment that contributed to establishing, developing, and maintaining the infrastructure.
A fee can be charged if the cost of the project exceeds 50 000 SEK. If assistance from infrastructure is required, an agreement has to be reached with the director of the infrastructure.

2. Industry users
A fee will be applied according to any of the service models below

We can provide a range of different levels of services for experienced and inexperienced users.

Consulting service model
In case consulting services are needed for setting up special experiment, we will arrange meetings involving the director, the senior researcher at the facility and the prospective user to discuss scientific questions and analysis needs. This will allow the user to choose the right instrumentation and protocol for their task. This service will be charged together with the services chosen by the user.

Training-based service model for inexperienced users
The facility offers high-content screening equipment for all users. We can provide theoretical and hands-on training to enable our users to use the equipment independently. The training cost will be calculated based on the working hours for the researcher or technician, and the instrument can be rented by the hour.

Full service model
A full service normally includes the whole process from experimental design to data delivery and analysis, whereby the facility provides user labs the possibility to outsource these skill and technology demanding screening projects. This service is subject to availability of our resources, and the pricing needs to be negotiated in advance. A collaboration model is recommended for such full services due to the demand on resources and personal. The collaboration model includes co-financing material cost, joint methodology development, joint data analysis and co-publication.

Query/sample analysis service model
For screening approaches which have already been set up in the facility (e.g. synthetic genetic array assay, Bioscreen assays, agar/liquid-based growth assay, fluorescent protein imaging-based screening). If the user can provide query strains that need to be analyzed, we offer to perform the analysis using our standard protocols. Basic data analysis will be performed after the screening. The cost will depend on consumables used and the working hours for the technician.

Consumables for purchase
We can offer a selection of consumables (plates, tips, pinning pads and reagent containers that are compatible with the setup of our instruments) for purchase. The items can be ordered through our website service.

All prices are subject to change without notice and are not guaranteed, except that prices for an order that have been accepted by ECLCS.

For high-content, high-throughput analysis
Staff
• Academic fee: 580 SEK / hour (excluding materials)
• Industry fee: 980 SEK / hour (excluding materials)

Equipment (high-content microscope and liquid handling robot)
• Academic fee: 450 SEK / hour (excluding materials)
• Industry fee: 850 SEK / hour (excluding materials)

For pinning robots
• Academic fee: 100 SEK / hour (excluding materials)
• Industry fee: 150 SEK / hour (excluding materials)

For Bioscreen
• Academic fee: 100 SEK / day (excluding materials)
• Industry fee: 150 SEK / day (excluding materials)

For scanning
• Academic fee: 100 SEK / day / scanner (excluding materials)
• Industry fee: 150 SEK / day / scanner (excluding materials)

The budget can typically be subdivided as follows:
1. The hours for staff to setup the high-content imaging-based screening and data analysis methods.
2. The number of hours for staff to perform the imaging-based screens.
3. The number of hours required for using the high-content image-based screening system.
4. The cost of materials needed (or provided by the user).