Center for Large-scale cell-based screening

The infrastructure consists of robots and automated phenotyping devices that are used in genome-wide approaches in many fields of bioscience. Software and databases have been developed for data generation, analysis, storage and presentation. Moreover, existing massive microbial strain collections are a fundament of the screening services that the infrastructure delivers.

Instrument and booking information 

• ImageXpress MICRO (MDC), automated cellular imaging and analysis system.
• MICROLAB STAR Line (Hamilton), large scale liquid handling robotic system.
• Synergy 2 Multi-Mode Microplate Reader (BioTek), to detect the cell density.
• Singer RoToR HDA (Singer Instrument), automated large-scale SGA analysis robot, to integrate reporters into different strain collections by cross recombination.
• Bioscreen C (OY Growth Curves AB), automated high throughput growth screening analysis.
• Flow cytometer, FACS Aria, BD equipment.

Services and prices

The facility consists of expert scientists, researchers and technicians that have extensive experience in different high-content screening fields, for example, agar-based screening, liquid-based profiling, imaging-based screening, etc. In general, the costs for our services will be calculated based on the instrument maintenance and depreciation, material and consumable and personnel costs. A collaboration model can be applied and depends on the agreement between the user and the specific researcher at the facility.

Services provided:

1. High-content, high throughput, imaging based screening (HCS)
2. High resolution, high-throughput quantitative growth phenotyping (liquid and solid medium growth phenotyping)
3. Synthetic gene arrays: genome-wide genetic interaction screens
4. Chemical genetics: bioactive molecules
5. Experimental evolution.