Laser Microdissection
Laser Microdissection and Pressure Catapulting (LMPC) is a non-contact sampling technique, especially for RNA, DNA and protein studies but also for cloning/live cell culture purposes. Microdissect a part of your specimen with a laser and catapult it into a collection device.
CCI has installed the new Leica Laser Microdissection (LMD) system which is a wide-field microscope-based platform designed for the precise isolation of specific cells or regions of interest (ROIs) from a wide range of biological samples using a contact-free sample collection method.
The Leica LMD system uses a pulsed laser to precisely cut around selected ROIs while minimizing damage to the surrounding tissue. The dissected material is then collected by gravity into a collection vessel or various types of well plates positioned under sample. This approach minimizes contamination and preserves the integrity of the isolated material. This technique is widely used in disease diagnostics, biological & biomaterial research, and biopharmaceutical development, such as genomics, proteomics, transcriptomics, and other molecular analyses. Scientists can isolate the targeted cells or complex structures from diverse biological samples, including tumor cells, neurons, sperm cells, specific vascular structures, and other anatomical features of interest. This enables more accurate sample analysis and reduces background signals from surrounding tissues.
Objectives list:
1: UVI 2.5X / 0.07
2: Plan 4X / 0.10NA , 26.2mm W.D.
3: HC PL FL 10X / 0.30
4: HC PL FL L 20X / 0.40 CORR
5: HC PL FLUOTAR L 40X / 0.60 CORR XT
6: HC PL FLUOTAR L 63X/ 0.70 CORR XT
7: HC PL APO 40X / 0.95 CORR
DNA
DNA research requires carefully selected source material, especially when studying individual cells, since PCR will amplify any material offered. Therefore, purest DNA is required to achieve reliable results. Cancer cells, for example, can be clearly separated from the surrounding tissue to allow contamination-free analysis and reproducible results.
RNA
Exactly separated analytical material is also important for the study of gene expression patterns. A prerequisite for that is to dissect and collect material preserving RNA in highest integrity. The best proof for the unparalleled quality of the LMPC method: even from single cells reliable gene expression analysis is possible. For all applications, only precise collected cells or tissues can yield precise and reproducible
results.
Proteins
Even in protein research, the trend is toward careful separation of individual cells or cell fractions. Cellular and tissue expressions induced by a wide
variety of factors can be determined when LMPC is used to harvest specimens at the protein level.
Living cells
An innovation in the field of laser microdissection is the isolation of live cells from tissue culture. You can isolate individual or small groups of cultured cells, even from primary cultures or stem cell preparations, can be used for direct molecular analysis or recultivation of single clones.
Laser microdissection and pressure catapulting combined with gene or protein analyses is useful in a wide range of research and clinical applications. The PALM® RoboMover allows this procedure in a high throughput performance by allowing up to 96 collection caps inserted at the same time. The software allows for automatic cutting-element detection and drawing through image analysis.
