Publications (MPE)
Since MPE started in 2004, proteins that we produced have been used in various studies that led to publications of the scientific work presented below.
2022
Persson Berg L, Eriksson M, Longhi S, Kockum I, Warnke C, Thomsson E, Bäckström M, Olsson T , Fogdell-Hahn A, Bergström T: Serum IgG levels to Epstein-Barr and measles viruses in patients with multiple sclerosis during natalizumab and interferon beta treatment. BMJ Neurol Open (2022) Jul 27;4(2):e000271 doi: 10.1136/bmjno-2022-000271.
Samuelsson E , Mirgorodskaya E , Nyström K , Bäckström M , Liljeqvist JÅ Nordén R: Sialic Acid and Fucose Residues on the SARS-CoV-2 Receptor-Binding Domain Modulate IgG Antibody Reactivity. ACS Infect Dis (2022). Aug 18 doi: 10.1021/acsinfecdis.2c00155.
2021
Jons D, Persson Berg L, Sundström P, Haghigi S, Axelsson M, Thulin M, Bergström T and Andersen O: Follow-up after infectious mononucleoises in search of serological similarities with presymptomatic multiple sclerosis. Multiple Sclerosis and Related Disorders (2021) 56:103288. https://doi.org/10.1016/j.msard.2021.103288
Görander S, Honda-Okubo Y, Bäckström M , Baldwin J, Bergström T , Petrovsky N, Liljeqvist JÅ: A truncated glycoprotein G vaccine formulated with Advax-CpG adjuvant provides protection of mice against genital herpes simplex virus 2 infection. Vaccine (2021) 39(40):5866-5875
Montoliu‐Gaya L, Tietze D, Kaminski D, Mirgorodskaya E, Tietze A and Sterky FH: CA10 regulates neurexin heparan sulfate addition via a direct binding in the secretory pathway. EMBO Rep. (2021) Apr 7; 22(4): e51349.
E. Ehrencrona, S. van der Post, P. Gallego, C. Recktenwald, A.M. Rodriguez-Pineiro, M.-J. Garcia-Bonete, S. Trillo-Muyo 1 , M. Bäckström, G.C Hansson, M.E.V. Johansson: The IgGFc-binding protein FCGBP is secreted with all GDPH sequences cleaved but maintained by interfragment disulfide bonds J Biol Chem (2021) 297(1):100871
2020
Marklund E, Leach S, Axelsson H, Nyström K, Norder H, Bemark M, Angeletti D, Lundgren A, Nilsson S, Andersson LM, Yilmaz A, Lindh M, Liljeqvist JÅ, Gisslén M: Serum-IgG responses to SARS-CoV-2 after mild and severe COVID-19 infection and analysis of IgG non-responders. PLOS One (2020) 15(10): e0241104. https://doi.org/10.1371/journal.pone.0241104
2019
Nordén R, Nilsson J , Samuelsson E, Risinger C, Sihlbom C , Blixt O, Larson G, Olofsson S and Bergström T: Recombinant Glycoprotein E of Varicella Zoster Virus Contains Glycan-Peptide Motifs That Modulate B Cell Epitopes into Discrete Immunological Signatures. Int J Mol Sci (2019) 20(4):954-
Nyström EEL, Arike L, Ehrencrona E, Hansson GC, Johansson MEV: Calcium-activated chloride channel regulator 1 (CLCA1) forms non-covalent oligomers in colonic mucus and has Mucin 2-processing properties. J Biol Chem. 2019 Sep 29. pii: jbc.RA119.009940. doi: 10.1074/jbc.RA119.009940. [Epub ahead of print]
2018
Nyström EEL, Birchenough GMH, van der Post S, Arike L, Gruber AD, Hansson GC, Johansson MEV: Calcium-activated Chloride Channel Regulator 1 (CLCA1) Controls Mucus Expansion in Colon by Proteolytic Activity. EBioMedicine. (2018) 33:134-143. doi: 10.1016/j.ebiom.2018.05.031.
Cervin J, Wands AM, Casselbrant A, Wu H, Krishnamurthy S, Cvjetkovic A, Estelius J, Dedic B, Sethi A, Wallom KL, Riise R, Bäckström M, Wallenius V, Platt FM, Lebens M, Teneberg S, Fändriks L, Kohler JJ, Yrlid U: GM1 ganglioside-independent intoxication by Cholera toxin. PLoS Pathog. (2018) Feb 12;14(2):e1006862. doi: 10.1371/journal.ppat.1006862
Trillo-Muyo S, Nilsson HE , Recktenwald CV, Ermund A, Ridley C, Meiss LN, Bähr A, Klymiuk N, Wine JJ, Koeck PJB, Thornton DJ, Hebert H, Hansson GC: Granule-stored MUC5B mucins are packed by the non-covalent formation of N-terminal head-to-head tetramers. J Biol Chem. (2018) Apr 13;293(15):5746-5754. doi: 10.1074/jbc.RA117.001014.
2017
Berglund AK, Navarrete C, Engqvist MKM, Hoberg E, Szilagyi Z, Taylor RW, Gustafsson CM, Maria Falkenberg M and Clausen AR: Nucleotide pools dictate the identity and frequency of ribonucleotide incorporation in mitochondrial DNA: PLOS Genetics (2017) http://dx.doi.org/10.1371/journal.pgen.1006628
2016
Bergström JH, Birchenough GMH, Katona G, Schroeder BO, Schütte A, Ermund A, Johansson MEV and Hansson GC: Gram-positive bacteria are held at a distance in the colon mucus by the lectin-like protein ZG16. Proc Natl Acad Sci U S A. (2016) Nov 29;113(48):13833-13838.
Recktenwald CV and Hansson GC: The Reduction-insensitive Bonds of the MUC2 Mucin Are Isopeptide Bonds. J. Biol. Chem. (2016) 291: 13580-. doi:10.1074/jbc.M116.726406
2014
Nilsson HE, Ambort D, Bäckström M, Thomsson E, Koeck PJB, Hansson GC and Hebert H: Intestinal MUC2 mucin supramolecular topology by packing and release resting on D3 domain assembly. J Mol Biol (2014) 54(2):250-6
2013
van der Post S, Subramani DB, Bäckström M, Johansson MEV, Vester-Christensen MB, Mandel U, Bennett EP, Clausen H, Dahlén G, Sroka A, Potempa J, and Hansson GC: Site-specific O-glycosylation on the MUC2 mucin inhibits cleavage by the Porphyromonas gingivalis secreted cysteine protease (RgpB) J Biol Chem (2013), 10.1074/jbc.M113.459479
Bäckström M, Ambort D, Thomsson E, Johansson MEV and Hansson GC: Increased understanding of the biochemistry and biosynthesis of MUC2 and other gel-forming mucin O-glycoproteins, through the recombinant expression of their protein domains. Mol Biotechnol (2013) 54(2):250-6
2012
B Wizel, K Thörn, E Nagy and AM Harandi: Nasal and skin delivery of IC31®-adjuvanted recombinant HSV-2 gD protein confers protection against genital herpes. Vaccine (2012) 30:4361-4368
Ambort D, Johansson ME, Gustafsson JK, Nilsson HE, Ermund A, Johansson BR, Koeck PJ, Hebert H, Hansson GC: Calcium and pH-dependent packing and release of the gel-forming MUC2 mucin. Proc Natl Acad Sci U S A. (2012) Apr 10;109(15):5645-50
Behnen P, Davis E, Delaney E, Frohm B, Bauer M, Cedervall T, O’Connell D, Åkerfeldt K and Linse S: Calcium-dependent interaction of calmodulin with Human 80S ribosomes and polyribosomes. Biochemistry (2012) 51:6718-6727
Chunseng J, Hultgård Ekwall AK, Bylund J, Björkman L, Estrella RP, Whitelock JM, Eisler T, Bokarewa M and Karlsson NG: Human synovial lubricin expresses sialyl Lewis X determinant and has L-selectin ligand activity. J Biol Chem (2012) 287:35922-35933
Wegmann F, Gartlan KH, Harandi AM, Brinckmann SA, Coccia M, Hillson WR, Kok WL, Cole S, Ho LP, Lambe T, Puthia M, Svanborg C, Scherer EM, Krashias G, Williams A, Blattman JN, Greenberg PD, Flavell RA, Moghaddam AE, Sheppard NC, Sattentau QJ. Polyethyleneimine is a potent mucosal adjuvant for viral glycoprotein antigens. Nat Biotechnol. (2012) Sep;30(9):883-8
2011
Ambort D, van der Post S, Johansson ME, Mackenzie J, Thomsson E, Krengel U, Hansson G. Function of the CysD domain of the gel-forming MUC2 mucin. Biochem J. (2011) 436:61-7
Thomsson E, Persson L, Grahn A, Snäll J, Ekblad M, Brunhage E, Svensson F, Jern C, Hansson GC, Bäckström M and Bergström T: Recombinant glycoprotein E produced in mammalian cells in large-scale as an antigen for varicella-zoster-virus serology (2011) 175:53-39
A Grahn, M Studahl, S Nilsson, E Thomsson, M Bäckström, and T Bergström: Varicella-zoster virus (VZV) glycoprotein E is a serological antigen for detection of intrathecal antibodies to VZV in central nervous system infections, without cross-reaction to herpes simplex virus type-1. Clin Vaccine Immunol (2011) 18:1336-1342
2010
AR Moslemi, C Lindberg, J Nilsson, H Tajshargi, B Andersson and A Oldfors: Glycogenin-1 deficiency and inactivated priming of glycogen synthesis. N Engl J Med (2010) 362:1203-10
2009
C Sihlbom, I van Dijk Härd, ME Lidell, T Noll, GC Hansson and M Bäckström: Localisation of O-glycans in recombinant MUC1 glycoproteins using electron-capture dissociation fragmentation mass spectrometry. Glycobiology (2009) 19:375-381
Lindqvist M, Persson J, Thörn K, Harandi AM. The mucosal adjuvant effect of alpha-galactosylceramide for induction of protective immunity to sexually transmitted viral infection. J Immunol. (2009) 182:6435-43
SE Domino, EA Hurd, KA Thomsson, DM Karnak, JM Holmén Larsson, E Thomsson, M Bäckström and GC Hansson: Cervical mucins carry α1,2 fucosylated glycans that partly protect from experimental vaginal candidiasis. Glycoconjugate J (2009) 26:1125-1134
J Del Campo, M Lindqvist, J Persson, M Bäckström, O Cabrerra, M Cuello, O Perez and AM Harandi: Intranasal immunization with a proteoliposome-derived cochleate containing recombinant gD protein confers protective immunity against genital herpes in mice. Vaccine (2009) 28:1193-1200
J Persson, M Bäckström, H Johansson, K Jirström, M Fernö, GC Hansson and M Ohlin: Molecular evolution of specific human antibody against MUC1 mucin results in improved recognition of the antigen on tumor cells. Tumour Biol (2009) 30(4):221-231
2007
E. Saeland, S. van Vliet, M. Bäckström, V.C.M. van den Berg, T.B.H. Geijtenbeek and Y. van Kooyk: The C-type lectin MGL expressed by dendritic cells detects glycan changes on MUC1 in colon carcinoma. Cancer Immunol Immunother (2007) 56:1225-1236