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A worldwide multicentre comparison of assays for cerebrospinal fluid biomarkers in Alzheimer's disease.

Artikel i vetenskaplig tidskrift
Författare N A Verwey
W M van der Flier
Kaj Blennow
C Clark
S Sokolow
P P De Deyn
D Galasko
H Hampel
T Hartmann
E Kapaki
L Lannfelt
P D Mehta
L Parnetti
A Petzold
T Pirttila
L Saleh
A Skinningsrud
J C V Swieten
M M Verbeek
J Wiltfang
S Younkin
P Scheltens
M A Blankenstein
Publicerad i Annals of clinical biochemistry
Volym 46
Nummer/häfte Pt 3
Sidor 235-40
ISSN 0004-5632
Publiceringsår 2009
Publicerad vid Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi
Sidor 235-40
Språk en
Länkar dx.doi.org/10.1258/acb.2009.008232
Ämnesord Alzheimer Disease, cerebrospinal fluid, Amyloid beta-Protein, cerebrospinal fluid, Biological Assay, methods, Enzyme-Linked Immunosorbent Assay, methods, Humans, Reproducibility of Results, tau Proteins, cerebrospinal fluid
Ämneskategorier Medicin och Hälsovetenskap

Sammanfattning

BACKGROUND: Different cerebrospinal fluid (CSF) amyloid-beta 1-42 (Abeta(1-42)), total Tau (Tau) and Tau phosphorylated at threonine 181 (P-Tau) levels are reported, but currently there is a lack of quality control programmes. The aim of this study was to compare the measurements of these CSF biomarkers, between and within centres. METHODS: Three CSF-pool samples were distributed to 13 laboratories in 2004 and the same samples were again distributed to 18 laboratories in 2008. In 2004 six laboratories measured Abeta(1-42), Tau and P-Tau and seven laboratories measured one or two of these marker(s) by enzyme-linked immunosorbent assays (ELISAs). In 2008, 12 laboratories measured all three markers, three laboratories measured one or two marker(s) by ELISAs and three laboratories measured the markers by Luminex. RESULTS: In 2004, the ELISA intercentre coefficients of variance (interCV) were 31%, 21% and 13% for Abeta(1-42), Tau and P-Tau, respectively. These were 37%, 16% and 15%, respectively, in 2008. When we restricted the analysis to the Innotest (N = 13) for Abeta(1-42), lower interCV were calculated (22%). The centres that participated in both years (N = 9) showed interCVs of 21%, 15% and 9% and intra-centre coefficients (intraCV) of variance of 25%,18% and 7% in 2008. CONCLUSIONS: The highest variability was found for Abeta(1-42). The variabilities for Tau and P-Tau were lower in both years. The centres that participated in both years showed a high intraCV comparable to their interCV, indicating that there is not only a high variation between but also within centres. Besides a uniform standardization of (pre)analytical procedures, the same assay should be used to decrease the inter/intracentre variation.

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