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An investigation of ribosomal protein L10 gene in autism spectrum disorders.

Artikel i vetenskaplig tidskrift
Författare Xiaohong Gong
Richard Delorme
Fabien Fauchereau
Christelle M Durand
Pauline Chaste
Catalina Betancur
Hany Goubran-Botros
Gudrun Nygren
Henrik Anckarsäter
Maria Råstam
I Carina Gillberg
Svenny Kopp
Marie-Christine Mouren-Simeoni
Christopher Gillberg
Marion Leboyer
Thomas Bourgeron
Publicerad i BMC Medical Genetics
Volym 10
Sidor 7
ISSN 1471-2350
Publiceringsår 2009
Publicerad vid Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi
Sidor 7
Språk en
Länkar dx.doi.org/10.1186/1471-2350-10-7
Ämnesord Autistic Disorder, Genetics, Chromosomes, Human, X, Cohort Studies, Exons, Female, Genetic Predisposition to Disease, Humans, Male, Mutation, RNA, Messenger, Genetics, Reverse Transcriptase Polymerase Chain Reaction, Ribosomal Proteins, Genetics, Sequence Analysis, DNA
Ämneskategorier Barn- och ungdomspsykiatri

Sammanfattning

BACKGROUND: Autism spectrum disorders (ASD) are severe neurodevelopmental disorders with the male:female ratio of 4:1, implying the contribution of X chromosome genetic factors to the susceptibility of ASD. The ribosomal protein L10 (RPL10) gene, located on chromosome Xq28, codes for a key protein in assembling large ribosomal subunit and protein synthesis. Two non-synonymous mutations of RPL10, L206M and H213Q, were identified in four boys with ASD. Moreover, functional studies of mutant RPL10 in yeast exhibited aberrant ribosomal profiles. These results provided a novel aspect of disease mechanisms for autism - aberrant processes of ribosome biosynthesis and translation. To confirm these initial findings, we re-sequenced RPL10 exons and quantified mRNA transcript level of RPL10 in our samples. METHODS: 141 individuals with ASD were recruited in this study. All RPL10 exons and flanking junctions were sequenced. Furthermore, mRNA transcript level of RPL10 was quantified in B lymphoblastoid cell lines (BLCL) of 48 patients and 27 controls using the method of SYBR Green quantitative PCR. Two sets of primer pairs were used to quantify the mRNA expression level of RPL10: RPL10-A and RPL10-B. RESULTS: No non-synonymous mutations were detected in our cohort. Male controls showed similar transcript level of RPL10 compared with female controls (RPL10-A, U=81, P=0.7; RPL10-B, U=61.5, P=0.2). We did not observe any significant difference in RPL10 transcript levels between cases and controls (RPL10-A, U=531, P=0.2; RPL10-B, U=607.5, P=0.7). CONCLUSION: Our results suggest that RPL10 has no major effect on the susceptibility to ASD.

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