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Determination of zeta-potential in rat organotypic hippocampal cultures.

Artikel i vetenskaplig tidskrift
Författare Yifat Guy
Mats Sandberg
Stephen G Weber
Publicerad i Biophysical journal
Volym 94
Nummer/häfte 11
Sidor 4561-9
ISSN 1542-0086
Publiceringsår 2008
Publicerad vid Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi
Sidor 4561-9
Språk en
Länkar dx.doi.org/10.1529/biophysj.107.112...
Ämnesord Animals, Biological Transport, Active, physiology, Cells, Cultured, Diffusion, Electromagnetic Fields, Hippocampus, chemistry, physiology, Microscopy, Fluorescence, methods, Rats
Ämneskategorier Cell- och molekylärbiologi

Sammanfattning

zeta-potentials of entities such as cells and synaptosomes have been determined, but zeta of brain tissue has never been measured. Electroosmotic flow, and the resulting transport of neuroactive substances, would result from naturally occurring and experimentally or clinically induced electric fields if zeta is significant. We have developed a simple method for determining zeta in tissue. An electric field applied across a rat organotypic hippocampal slice culture (OHSC) drives fluorescent molecules through the tissue by both electroosmotic flow and electrophoresis. Fluorescence microscopy is used to determine each molecule's velocity. Independently, capillary electrophoresis is used to measure the molecules' electrophoretic mobilities. The experiment yields zeta-potential and average tissue tortuosity. The zeta-potential of OHSCs is -22 +/- 2 mV, and the average tortuosity is 1.83 +/- 0.06. In a refined experiment, zeta-potential is measured in various subregions. The zeta-potentials of the CA1 stratum pyramidale, CA3 stratum pyramidal, and dentate gyrus are -25.1 +/- 1.6 mV, -20.3 +/- 1.7 mV, and -25.4 +/- 1.0 mV, respectively. Simple dimensional arguments show that electroosmotic flow is potentially as important as diffusion in molecular transport.

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