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Reduced diversity in the early fecal microbiota of infants with atopic eczema.

Artikel i vetenskaplig tidskrift
Författare Mei Wang
Caroline Karlsson
Crister Olsson
Ingegerd Adlerberth
Agnes E Wold
David P. Strachan
Paolo M. Martricardi
Nils Åberg
Michael R. Perkin
Salvatore Tripodi
Anthony R Coates
Bill Hesselmar
Robert Saalman
Göran Molin
Siv Ahrné
Publicerad i Journal of Allergy and Clinical Immunology
Volym 121
Nummer/häfte 1
Sidor 129-134
ISSN 1097-6825
Publiceringsår 2008
Publicerad vid Institutionen för biomedicin, avdelningen för infektionssjukdomar
Institutionen för kliniska vetenskaper
Sidor 129-134
Språk en
Länkar dx.doi.org/10.1016/j.jaci.2007.09.0...
Ämnesord Atopic eczema; intestinal microbiota; diversity; terminal restriction fragment length polymorphism; temporal temperature gradient gel electrophoresis
Ämneskategorier Immunologi inom det medicinska området

Sammanfattning

BACKGROUND: It might be that early intestinal colonization by bacteria in westernized infants fails to give rise to sufficient immune stimulation to support maturation of regulatory immune mechanisms. OBJECTIVE: The purpose of the present study was to characterize the very early infantile microbiota by using a culture-independent approach and to relate the colonization pattern to development of atopic eczema in the first 18 months of life. METHODS: Fecal samples were collected from 35 infants at 1 week of age. Twenty infants were healthy, and 15 infants were given diagnoses of atopic eczema at the age of 18 months. The fecal microbiota of the infants was compared by means of terminal restriction fragment length polymorphism (T-RFLP) and temporal temperature gradient gel electrophoresis (TTGE) analysis of amplified 16S rRNA genes. RESULTS: By means of T-RFLP analysis, the median number of peaks, Shannon-Wiener index, and Simpson index of diversity were significantly less for infants with atopic eczema than for infants remaining healthy in the whole group and for the Swedish infants when AluI was used for digestion. The same was found when TTGE patterns were compared. In addition, TTGE analysis showed significantly less bands and lower diversity indices for the British atopic infants compared with those of the control subjects. CONCLUSION: There is a reduced diversity in the early fecal microbiota of infants with atopic eczema during the first 18 months of life.

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