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Rotavirus infection is not associated with small intestinal fluid secretion in the adult mouse.

Artikel i vetenskaplig tidskrift
Författare Shirin Kordasti
Claudia Istrate
Mahanez Banasaz
Martin Rottenberg
Henrik Sjövall
Ove Lundgren
Lennart Svensson
Publicerad i Journal of virology
Volym 80
Nummer/häfte 22
Sidor 11355-61
ISSN 0022-538X
Publiceringsår 2006
Publicerad vid Institutionen för neurovetenskap och fysiologi, sektionen för fysiologi
Institutionen för medicin, avdelningen för invärtesmedicin
Sidor 11355-61
Språk en
Länkar dx.doi.org/10.1128/JVI.00152-06
Ämnesord Animals, Body Fluids, secretion, Chlorides, metabolism, Disease Models, Animal, Duodenum, secretion, virology, Feces, virology, Gastrointestinal Motility, Histocytochemistry, Ileum, secretion, virology, Immunohistochemistry, Intestinal Mucosa, secretion, Intestine, Small, pathology, physiopathology, secretion, virology, Jejunum, secretion, virology, Mice, Mice, Inbred BALB C, Organ Size, RNA, Viral, analysis, Rotavirus Infections, physiopathology, virology
Ämneskategorier Fysiologi, Mikrobiologi inom det medicinska området

Sammanfattning

In contrast to humans, adult but not infant small animals are resistant to rotavirus diarrhea. The pathophysiological mechanism behind this age-restricted diarrhea is currently unresolved, and this question was investigated by studying the secretory state of the small intestines of adult mice infected with rotavirus. Immunohistochemistry and histological examinations revealed that rotavirus (strain EDIM) infects all parts of the small intestines of adult mice, with significant numbers of infected cells in the ilea at 2 and 4 days postinfection. Furthermore, quantitative PCR revealed that 100-fold more viral RNA was produced in the ilea than in the jejuna or duodena of adult mice. In vitro perfusion experiments of the small intestine did not reveal any significant changes in net fluid secretion among mice infected for 3 days or 4 days or in those that were noninfected (37 +/- 9 microl . h(-1) . cm(-1), 22 +/- 13 microl . h(-1) . cm(-1), and 33 +/- 6 microl . h(-1) . cm(-1), respectively) or in transmucosal potential difference (4.0 +/- 0.3 mV versus 3.9 +/- 0.4 mV), a marker for active chloride secretion, between control and rotavirus-infected mice. In vivo experiments also did not show any differences in potential difference between uninfected and infected small intestines. Furthermore, no significant differences in weight between infected and uninfected small intestines were found, nor were any differences in fecal output observed between infected and control mice. Altogether, these data suggest that rotavirus infection is not sufficient to stimulate chloride and water secretion from the small intestines of adult mice.

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