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Down-regulation of the sodium/iodide symporter explains 131I-induced thyroid stunning.

Artikel i vetenskaplig tidskrift
Författare Madeleine Nordén
Fredrik Larsson
Sofia Tedelind
Therese Carlsson
Charlotta Lundh
Eva Forssell-Aronsson
Mikael Nilsson
Publicerad i Cancer research
Volym 67
Nummer/häfte 15
Sidor 7512-7
ISSN 0008-5472
Publiceringsår 2007
Publicerad vid Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi
Institutionen för kliniska vetenskaper
Sidor 7512-7
Språk en
Länkar dx.doi.org/10.1158/0008-5472.CAN-07...
Ämnesord Animals, Down-Regulation, Insulin-Like Growth Factor I, pharmacology, Iodine Radioisotopes, adverse effects, RNA, Messenger, genetics, metabolism, Radiation Injuries, etiology, prevention & control, Reverse Transcriptase Polymerase Chain Reaction, Swine, Symporters, antagonists & inhibitors, genetics, metabolism, Thyroid Diseases, etiology, prevention & control, radionuclide imaging, Thyroid Gland, injuries, radiation effects, radionuclide imaging, Thyrotropin, pharmacology
Ämneskategorier Medicin och Hälsovetenskap

Sammanfattning

(131)I radiation therapy of differentiated thyroid cancer may be compromised by thyroid stunning (i.e., a paradoxical inhibition of radioiodine uptake caused by radiation from a pretherapeutic diagnostic examination). The stunning mechanism is yet uncharacterized at the molecular level. We therefore investigated whether the expression of the sodium/iodide symporter (NIS) gene is changed by irradiation using (131)I. Confluent porcine thyroid cells on filter were stimulated with thyroid-stimulating hormone (TSH; 1 milliunit/mL) or insulin-like growth factor-I (IGF-I; 10 ng/mL) and simultaneously exposed to (131)I in the culture medium for 48 h, porcine NIS mRNA was quantified by real-time reverse transcription-PCR using 18S as reference, and transepithelial iodide transport was monitored using (125)I(-) as tracer. TSH increased the NIS expression >100-fold after 48 h and 5- to 20-fold after prolonged stimulation. IGF-I enhanced the NIS transcription at most 15-fold but not until 5 to 7 days. (131)I irradiation (7.5 Gy) decreased both TSH-stimulated and IGF-I-stimulated NIS transcription by 60% to 90% at all investigated time points. TSH and IGF-I stimulated NIS synergistically 15- to 60-fold after 5 days. NIS expression was reduced by (131)I also in costimulated cells, but the transcription level remained higher than in nonirradiated cells stimulated with TSH alone. Changes in NIS mRNA always correlated with altered (125)I(-) transport in cultures with corresponding treatments. It is concluded that down-regulation of NIS is the likely explanation of (131)I-induced thyroid stunning. Enhanced NIS expression by synergistically acting agents (TSH and IGF-I) partly prevents the loss of iodide transport expected from a given absorbed dose, suggesting that thyroid stunning might be pharmacologically treatable.

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