Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän
Utskriftsversion

Localization of lipids in… - Göteborgs universitet Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

Localization of lipids in the aortic wall with imaging TOF-SIMS

Artikel i vetenskaplig tidskrift
Författare Per Malmberg
K. Borner
Yun Chen
Peter Friberg
B. Hagenhoff
Jan-Eric Månsson
Håkan Nygren
Publicerad i Biochim Biophys Acta
Volym 1771
Nummer/häfte 2
Sidor 185-95
ISSN 0006-3002 (Print)
Publiceringsår 2007
Publicerad vid Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi
Institutionen för medicin, avdelningen för molekylär och klinisk medicin
Sidor 185-95
Språk en
Länkar www.ncbi.nlm.nih.gov/entrez/query.f...
Ämnesord Animals, Aorta/*metabolism/ultrastructure, Atherosclerosis/metabolism, Cholesterol/metabolism, Fatty Acids, Monounsaturated/metabolism, Freeze Drying, Humans, Lipids/*analysis, Oleic Acid/metabolism, Palmitic Acid/metabolism, Rats, Rats, Sprague-Dawley, Spectrometry, Mass, Secondary Ion/*methods, Stearic Acids/metabolism
Ämneskategorier Medicin och Hälsovetenskap

Sammanfattning

Time-of-flight secondary-ion-mass-spectrometry (TOF-SIMS) was utilized to address the issue of localization of lipids and inorganic ions in healthy rat aorta and human atherosclerotic plaque. Pieces of rat aorta were high pressure frozen, freeze-fractured and freeze dried. The samples were analyzed by imaging TOF-SIMS equipped with a Bi(1-7)(+)-source. Reference lipid samples were analyzed and compared to data obtained by analysis of the rat aorta samples. Fatty acids, cholesterol, oxysterol and diacylglycerols were detected and localized. A heterogeneous lipid distribution could be shown in the aorta, where the lamellae of the aorta, distinguished by imaging of CN(-), appeared enriched in cholesterol, oxysterol and diacylglycerols, while the smooth muscle tissue, identified by imaging of PO(3), appeared enriched in phosphocholine. Palmitic/palmitoleic acid and stearic/oleic acid appeared to be heterogeneously distributed over the aorta with high concentration areas located especially in the tunica media region of the aorta. Human atherosclerotic plaque showed an irregular cholesterol distribution mainly located in spots in the intima region with elongated diacylglycerol regions located mainly in the media region.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?