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Shedding and gamma-Secretase mediated intramembrane proteolysis of the mucin-type molecule CD43

Artikel i vetenskaplig tidskrift
Författare Christian X Andersson
Julia Fernandez-Rodriguez
Sirle Laos
Dan Baeckström
Gunnar C. Hansson
Christian Haass
Publicerad i Biochem J.
Volym 387
Nummer/häfte 2
Sidor 377-384
Publiceringsår 2005
Publicerad vid Institutionen för medicinsk och fysiologisk kemi
Sidor 377-384
Språk en
Länkar dx.doi.org/10.1042/BJ20041387.
Ämneskategorier Biologiska vetenskaper, Cell- och molekylärbiologi

Sammanfattning

CD43 is a transmembrane molecule that contains a 123-aminoacids-long cytoplasmic tail and a highly O-glycosylated extracellular domain of mucin type. Endogenous CD43 expressed in COLO 205, K562 and Jurkat cells revealed a membrane-associated, 20 kDa CD43-specific cytoplasmic tail fragment (CD43-CTF) upon inhibition of gamma-secretase. This fragment was formed by an extracellular cleavage, as it was not accumulated after treating cells with 1,10-phenanthroline, a metalloprotease inhibitor. When CD43 was transfected into HEK-293 cells expressing dominant-negative PS1 (presenilin-1), the CD43-CTF was accumulated, but not in cells with wild-type PS1. Owing to its accumulation in the presence of a non-functional PS variant, it may thus be a novel gamma-secretase substrate. This CTF is formed by an extracellular cleavage close to the membrane, is a fragment that can be concluded to be a substrate for gamma-secretase. However, the intracellular gamma-secretase product has not been possible to detect, suggesting a quick processing of this product. During normal growth the CTF was not found without gamma-secretase inhibition, but when the cells (COLO 205) were very confluent the fragment could be detected. The intracellular domain of CD43 has previously been shown to contain a functional nuclear localization signal, and has been suggested to be involved in gene activation. From this and the present results, a novel way to explain how mucin-type molecules may transduce intracellular signals can be proposed.

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