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T-cell costimulatory capacity of oral and skin epithelial cells in vitro: presence of suppressive activity in supernatants from skin epithelial cell cultures.

Artikel i vetenskaplig tidskrift
Författare Bengt Hasséus
Mats Jontell
Gunnar Bergenholtz
Ulf Dahlgren
Publicerad i European journal of oral sciences
Volym 112
Nummer/häfte 1
Sidor 48-54
ISSN 0909-8836
Publiceringsår 2004
Publicerad vid Institutionen för invärtesmedicin, Avdelningen för reumatologi och inflammationsforskning
Odontologiska institutionen, Avdelningen för endodonti med oral diagnostik
Sidor 48-54
Språk en
Länkar www.ncbi.nlm.nih.gov/entrez/query.f...
Ämnesord Animals, Cell Separation, Cells, Cultured, Culture Media, Conditioned, pharmacology, Female, Flow Cytometry, Histocompatibility Antigens Class II, biosynthesis, Interferon Type II, pharmacology, Langerhans Cells, drug effects, immunology, metabolism, Lymphocyte Activation, Lymphocyte Culture Test, Mixed, Mouth Mucosa, cytology, immunology, Rats, Rats, Inbred Lew, Rats, Wistar, Skin, cytology, immunology, Suppressor Factors, Immunologic, T-Lymphocytes, drug effects, immunology
Ämneskategorier Odontologi

Sammanfattning

Oral Langerhans cells (LC) have better T-cell costimulatory capacity than skin LC. In this study factors affecting this capacity have been assessed in a mixed epithelial cell lymphocyte reaction (MELR) assay. Flow cytometry analysis of freshly recovered cells revealed major histocompatibility complex (MHC) class II molecule expression on 7.5% of the oral epithelial cells and 9.7% of the skin epithelial cells. Monoclonal anti class II antibodies significantly reduced the T-cell proliferation in the MELR. Pretreatment of skin epithelial cells with interleukin-1beta, tumour necrosis factor-alpha or interferon (IFN)-gamma did not affect the MELR proliferation, but incubation with IFNgamma significantly suppressed the T-cell response. Transfer of supernatants from cultures of skin epithelial cells and allogeneic T cells to cultures of oral epithelial cells and T cells resulted in a reduced T-cell proliferation while supernatants from oral epithelial cells and T cells did not reduce proliferation. The higher proliferation in cultures of T cells and oral epithelial cells than in cultures containing skin epithelial cells may be due to the presence of a suppressive factor in the skin epithelial cell suspensions.

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