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Impaired regulatory T cell function in germ-free mice.

Artikel i vetenskaplig tidskrift
Författare Sofia M Östman
Carola Rask
Agnes E Wold
Susanne Hultkrantz
Esbjörn Telemo
Publicerad i European journal of immunology
Volym 36
Nummer/häfte 9
Sidor 2336-46
ISSN 0014-2980
Publiceringsår 2006
Publicerad vid Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning
Institutionen för biomedicin, avdelningen för infektionssjukdomar
Sidor 2336-46
Språk en
Länkar dx.doi.org/10.1002/eji.200535244
Ämnesord Animals, Antigens, CD, Antigens, CD4, biosynthesis, immunology, Antigens, Differentiation, biosynthesis, immunology, Flow Cytometry, Forkhead Transcription Factors, biosynthesis, immunology, Gene Expression, Germ-Free Life, Immune Tolerance, Interleukin-10, biosynthesis, Interleukin-13, biosynthesis, Lymph Nodes, cytology, immunology, Mice, Phenotype, RNA, Messenger, analysis, Receptors, Interleukin-2, biosynthesis, immunology, T-Lymphocyte Subsets, immunology, T-Lymphocytes, Regulatory, immunology
Ämneskategorier Medicin och Hälsovetenskap

Sammanfattning

Regulatory T cells (Treg) are crucial for the maintenance of tolerance to auto-antigens and harmless exogenous antigens. Here, we studied the role of the commensal microbiota for the development and function of Treg. CD4+CD25+ T cells were obtained from peripheral lymph nodes (PLN) and mesenteric lymph nodes (MLN) of germ-free (GF) and conventional (conv) NMRI mice and tested for phenotype and functional suppressive capacity. CD4+CD25+ T cells from GF mice showed a lower relative gene expression of fork head box p3 gene (Foxp3) and were not as potent suppressors in vitro as CD4+CD25+ T cells from conv animals. Intracellular staining for Foxp3 and CTLA-4 revealed proportional and regional differences in putative Treg subsets between conv and GF mice. Fewer of the CD4+CD25+ T cells in GF MLN expressed Foxp3 and CTLA-4, while the expression of these markers was similar amongst the CD4+CD25+ T cells in PLN of conv and GF mice. The largest difference between conv and GF Treg was observed in the liver draining celiac lymph node, where GF mice had fewer putative Treg as compared to conv mice. We propose that the presence of a microbial flora favors the development of a fully functional Treg population.

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