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Gram-positive and Gram-negative bacteria elicit different patterns of pro-inflammatory cytokines in human monocytes.

Artikel i vetenskaplig tidskrift
Författare Christina Hessle
Bengt A. Andersson
Agnes E Wold
Publicerad i Cytokine
Volym 30
Nummer/häfte 6
Sidor 311-8
ISSN 1043-4666
Publiceringsår 2005
Publicerad vid Institutionen för laboratoriemedicin, Avdelningen för klinisk immunologi
Institutionen för laboratoriemedicin, Avdelningen för klinisk bakteriologi
Sidor 311-8
Språk en
Länkar dx.doi.org/10.1016/j.cyto.2004.05.0...
Ämnesord Cell Wall, metabolism, Cytokines, metabolism, Dose-Response Relationship, Drug, Enzyme-Linked Immunosorbent Assay, Escherichia coli, metabolism, Gram-Negative Bacteria, metabolism, Gram-Positive Bacteria, metabolism, Humans, Inflammation, Interleukin-1, metabolism, Interleukin-6, metabolism, Interleukin-8, metabolism, Kinetics, Monocytes, metabolism, microbiology, Time Factors, Tumor Necrosis Factor-alpha, metabolism, Ultraviolet Rays
Ämneskategorier Medicin och Hälsovetenskap

Sammanfattning

Pro-inflammatory cytokines secreted by tissue macrophages recruit polymorphonuclear leukocytes and evoke fever, cachexia and production of acute phase proteins. This study investigates whether Gram-positive and Gram-negative bacteria equally and efficiently trigger production of the pro-inflammatory cytokines IL-1 beta, IL-6, IL-8 and TNF-alpha in human monocytes. A range of aerobic and anaerobic Gram-positive and Gram-negative bacteria were killed by UV-light and added in different concentrations to human monocytes. Cytokines were measured in 24 h supernatants by ELISA. Gram-positive and Gram-negative bacteria were equally efficient inducers of IL-1 beta, but Gram-positive bacteria generated twice as much TNF-alpha as did Gram-negative bacteria (p<0.001 for 25 and 250 bacteria/cell). In contrast, Gram-negative bacteria induced at least twice as much IL-6 and IL-8 as did Gram-positive bacteria (p<0.001 for 2.5, 25 and 250 bacteria/cell). While the cytokine responses to LPS were similar to those induced by the corresponding amount of Gram-negative bacteria, the strong IL-1 beta and TNF-alpha responses to Gram-positive bacteria could not be induced by soluble peptidoglycan or lipotheicoic acid. The particular nature of the bacteria, thus seem to modify the response to Gram-positive bacterial components. The different cytokine profiles evoked by Gram-positive and Gram-negative bacteria might optimize clearance of bacteria that differ in cell wall structure.

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