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Relation of the size and intracellular sorting of apoB to the formation of VLDL 1 and VLDL 2

Artikel i vetenskaplig tidskrift
Författare Pia Stillemark-Billton
Caroline Beck
Jan Borén
Sven-Olof Olofsson
Publicerad i J Lipid Res
Volym 46
Nummer/häfte 1
Sidor 104-14
ISSN 0022-2275 (Print)
Publiceringsår 2005
Publicerad vid Wallenberglaboratoriet
Sidor 104-14
Språk en
Länkar www.ncbi.nlm.nih.gov/entrez/query.f...
Ämnesord Animals, Apolipoprotein B-100, Apolipoproteins B/*metabolism, Cell Line, Tumor, Immunoprecipitation, Lipoproteins, VLDL/*biosynthesis, Liver/cytology, Mice, Molecular Chaperones, Particle Size, Protein Transport, Rats, Transfection, Triglycerides/metabolism, Ultracentrifugation
Ämneskategorier Medicin och Hälsovetenskap

Sammanfattning

In this study, we tested the hypothesis that two separate pathways, the two-step process and an apolipoprotein B (apoB) size-dependent lipidation process, give rise to different lipoproteins. Expression of apoB-100 and C-terminally truncated forms of apoB-100 in McA-RH7777 cells demonstrated that VLDL particles can be assembled by apoB size-dependent linear lipidation, resulting in particles whose density is inversely related to the size of apoB. This lipidation results in a LDL-VLDL 2 particle containing apoB-100. VLDL 1 is assembled by the two-step process by apoB-48 and larger forms of apoB but not to any significant amount by apoB-41. The major amount of intracellular apoB-80 and apoB-100 banded with a mean density of 1.10 g/ml. Its formation was dependent on the sequence between apoB-72 and apoB-90. This dense particle, which is retained in the cell, possibly by chaperones or association with the microsomal membrane, is a precursor of secreted VLDL 1. The intracellular LDL-VLDL 2 particles formed during size-dependent lipidation appear to be the precursors of intracellular VLDL 1. We propose that the dense apoB-100 intracellular particle is converted to LDL-VLDL 2 by size-dependent lipidation. LDL-VLDL 2 is secreted or converted to VLDL 1 by the uptake of the major amount of triglycerides.

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