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In vivo MR imaging of magnetically labeled human embryonic stem cells

Artikel i vetenskaplig tidskrift
Författare Tommi Tallheden
Ulf Nannmark
Malin Lorentzon
O. Rakotonirainy
Bassam Soussi
Finn Waagstein
Anders Jeppsson
E. Sjogren-Jansson
Anders Lindahl
Elmir Omerovic
Publicerad i Life sciences
Volym 79
Nummer/häfte 10
Sidor 999-1006
ISSN 0024-3205 (Print)
Publiceringsår 2006
Publicerad vid Wallenberglaboratoriet
Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin
Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi
Institutionen för medicin, avdelningen för molekylär och klinisk medicin
Institutionen för kliniska vetenskaper
Sidor 999-1006
Språk en
Länkar www.ncbi.nlm.nih.gov/entrez/query.f...
Ämnesord Animals, Cell Differentiation/drug effects, Cell Proliferation/drug effects, Cell Survival/drug effects, Feasibility Studies, Heart/anatomy & histology, Humans, Iron/*pharmacokinetics, *Magnetic Resonance Imaging, *Magnetics, Oxides/*pharmacokinetics, Rats, Staining and Labeling, *Stem Cell Transplantation/methods, Stem Cells/*cytology/drug effects/*metabolism, Transplantation, Heterologous
Ämneskategorier Medicin och Hälsovetenskap

Sammanfattning

INTRODUCTION: Human embryonic stem cells (hES) have emerged as a potentially new therapeutic approach for treatment of heart and other diseases applying the concept of regenerative medicine. A method for in vivo visualization and tracking of transplanted hES would increase our understanding of in vivo hES behavior in both experimental and clinical settings. The aim of this study was to evaluate the feasibility of magnetic labeling and visualization of hES with magnetic resonance imaging (MRI). METHODS: hES were established and expanded according to standard procedures. After expansion, the cells were cultured under feeder free conditions and magnetically labeled by addition of dextran-coated Ferrum-oxide particles (Endorem) to the medium. Accumulation of small particles of iron-oxide (SPIO) in hES was assessed by Prussian blue staining and electron microscopy. For in vitro MRI, the labeled and unlabeled hES were examined in cell solution and after transplantation into explanted mouse heart ( approximately 100,000 cells) on a Bruker Avance DMX 500 vertical magnet at 11.75 T. A multi-slice, multi spin-echo T(2)-weighted images were obtained. For in vivo imaging, the experiments were performed on male Sprague-Dawley using Bruker Biospec 2.35 T magnet. The hES were directly injected ( approximately 500,000 cells) after surgical procedure (thoracotomy) into anterior left ventricular (LV) wall. Multi-slice T(2)-weighted gradient echo images were obtained using cardiac gating. RESULTS: hES appeared to be unaffected by magnetic labeling and maintained their ability to proliferate and differentiate. No additive agent for membrane permeabilisation was needed for facilitation of intracellular SPIO accumulation. Prussian blue and electron microscopy have revealed numerous iron particles in the cytoplasm of hES. On T(2)-weighted images, the labeled cells have shown well-defined hyopintense areas at the site of injection in anterior LV wall both in vitro and in vivo. CONCLUSIONS: It is feasible to magnetically label and visualize hES both in vitro and in vivo. MR visualization of magnetically labeled hES may be a valuable tool for in vivo tracking of hES.

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