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Increased MUC1 plus a larger quantity and complex size for MUC5AC in the peripheral airway lumen of long-term tobacco smokers

Artikel i vetenskaplig tidskrift
Författare Médea Padra
Anders Andersson
B. Levanen
Pushpa Premaratne
Helga Asgeirsdottir
Sara Tengvall
Karin Christenson
Marit Stockfelt
S. Bozinovski
S. Yoshihara
A. S. Lantz
L. Palmberg
B. Dahlen
K. Larsson
Ingemar Qvarfordt
Sara K. Lindén
A. Linden
Publicerad i Clinical Science
Volym 134
Nummer/häfte 10
Sidor 1107-1125
ISSN 0143-5221
Publiceringsår 2020
Publicerad vid Institutionen för odontologi
Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition
Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi
Sidor 1107-1125
Språk en
Länkar dx.doi.org/10.1042/cs20191085
Ämnesord obstructive pulmonary-disease, inflammatory cells, mucins muc5ac, hypersecretion, expression, mortality, persistent, lavage, sputum, phase, Research & Experimental Medicine
Ämneskategorier Lungmedicin och allergi

Sammanfattning

There is little information on mucins versus potential regulatory factors in the peripheral airway lumen of long-term smokers with (LTS+) and without (LTS-) chronic obstructive pulmonary disease (COPD). We explored these matters in bronchoalveolar lavage (BAL) samples from two study materials, both including LTS+ and LTS- with a very similar historic exposure to tobacco smoke, and healthy non-smokers (HNSs; n=4-20/group). Utilizing slot blot and immunodetection of processed (filtered and centrifuged), as well as unprocessed BAL samples from one of the materials, we compared the quantity and fraction of large complexes of mucins. All LTS displayed an enhanced (median) level of MUC5AC compared with HNS. LTS- displayed a higher level of large MUC5AC complexes than HNS while LTS+ displayed a similar trend. In all LTS, total MUC5AC correlated with blood leukocytes, BAL neutrophil elastase and net gelatinase activity. Large mucin complexes accounted for most MUC5B, without clear group differences. In all LTS, total MUC5B correlated with total MUC5AC and local bacteria. In the same groups, large MUC5B complexes correlated with serum cotinine. MUC1 was increased and correlated with BAL leukocytes in all LTS whereas MUC2 was very low and without clear group differences. Thus, the main part of MUC5AC and MUC5B is present as large complexes in the peripheral airway lumen and historic as well as current exposure to tobacco smoke emerge as potential regulatory factors, regardless of COPD per se. Bacteria, leukocytes and proteinases also constitute potential regulatory factors, of interest for future therapeutic strategies.

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