Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän
Utskriftsversion

Patient-derived scaffolds… - Göteborgs universitet Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

Patient-derived scaffolds uncover breast cancer promoting properties of the microenvironment

Artikel i vetenskaplig tidskrift
Författare Göran Landberg
Paul A. Fitzpatrick
Pauline Isakson
Emma Jonasson
Joakim Karlsson
E. Larsson
Andreas Svanström
Svanheidur Rafnsdottir
Emma Persson
Anna Gustafsson
Daniel Andersson
J. Rosendahl
S. Petronis
Parmida Ranji
Pernilla Gregersson
Ylva Magnusson
J. Hakansson
Anders Ståhlberg
Publicerad i Biomaterials
Volym 235
ISSN 0142-9612
Publiceringsår 2020
Publicerad vid Institutionen för biomedicin, avdelningen för laboratoriemedicin
Wallenberg Centre for Molecular and Translational Medicine
Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi
Språk en
Länkar dx.doi.org/10.1016/j.biomaterials.2...
Ämnesord Breast cancer, Cancer stem cells, Differentiation, Infiltration, Malignancy, Scaffold, tumor xenografts, tissue scaffold, in-vitro, stem, expression, models, cells, hallmarks, culture, 3rd-dimension, Engineering, Materials Science
Ämneskategorier Cancer och onkologi

Sammanfattning

Tumor cells interact with the microenvironment that specifically supports and promotes tumor development. Key components in the tumor environment have been linked to various aggressive cancer features and can further influence the presence of subpopulations of cancer cells with specific functions, including cancer stem cells and migratory cells. To model and further understand the influence of specific microenvironments we have developed an experimental platform using cell-free patient-derived scaffolds (PDSs) from primary breast cancers infiltrated with standardized breast cancer cell lines. This PDS culture system induced a series of orchestrated changes in differentiation, epithelial-mesenchymal transition, stemness and proliferation of the cancer cell population, where an increased cancer stem cell pool was confirmed using functional assays. Furthermore, global gene expression profiling showed that PDS cultures were similar to xenograft cultures. Mass spectrometry analyses of cell-free PDSs identified subgroups based on their protein composition that were linked to clinical properties, including tumor grade. Finally, we observed that an induction of epithelial-mesenchymal transition-related genes in cancer cells growing on the PDSs were significantly associated with clinical disease recurrences in breast cancer patients. Patient-derived scaffolds thus mimics in vivo-like growth conditions and uncovers unique information about the malignancy-inducing properties of tumor microenvironment.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?