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Vesicular Transmitter Content in Chromaffin Cells Can Be Regulated via Extracellular ATP

Artikel i vetenskaplig tidskrift
Författare Anna Larsson
Soodabeh Majdi
R. Borges
Andrew G Ewing
Publicerad i ACS Chemical Neuroscience
Volym 10
Nummer/häfte 11
Sidor 4735-4740
ISSN 1948-7193
Publiceringsår 2019
Publicerad vid Institutionen för kemi och molekylärbiologi
Sidor 4735-4740
Språk en
Länkar dx.doi.org/10.1021/acschemneuro.9b0...
Ämnesord ATP, amperometry, intracellular vesicle impact cytometry, exocytosis, vesicle content, catecholamine uptake, quantal size, exocytosis, chromogranins, storage, vesicles, release, norepinephrine, accumulation, pharmacology, Biochemistry & Molecular Biology, Pharmacology & Pharmacy, Neurosciences, & Neurology
Ämneskategorier Biokemi och molekylärbiologi

Sammanfattning

The energy carrying molecule adenosine triphosphate (ATP) has been implicated for its role in modulation of chemical signaling for some time. Despite this, the precise effects and mechanisms of action of ATP on secretory cells are not well-known. Here, bovine chromaffin cells have been used as a model system to study the effects of extracellular ATP in combination with the catecholamine transmitter norepinephrine (NE). Both transmitter storage and exocytotic release were quantified using complementary amperometric techniques. Although incubation with NE alone did not cause any changes to either transmitter storage or release, coincubation with NE and ATP resulted in a significant increase that was concentration dependent. To probe the potential mechanisms of action, a slowly hydrolyzable version of ATP, ATP-gamma-S, was used either alone or together with NE. The result implicates two different behaviors of ATP acting on both the purinergic autoreceptors and as a source of the energy needed to load chromaffin cell vesicles.

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