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Human cerebrospinal fluid promotes spontaneous gamma oscillations in the hippocampus in vitro

Artikel i vetenskaplig tidskrift
Författare Andreas Björefeldt
F. Roshan
My Forsberg
Henrik Zetterberg
Eric Hanse
A. Fisahn
Publicerad i Hippocampus
ISSN 1050-9631
Publiceringsår 2019
Publicerad vid Institutionen för neurovetenskap och fysiologi
Institutionen för neurovetenskap och fysiologi, sektionen för fysiologi
Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi
Språk en
Länkar dx.doi.org/10.1002/hipo.23135
Ämnesord CSF, fast-spiking interneuron, hippocampal brain slice, neuromodulation, pyramidal cell, action-potential desynchronization, fast network oscillations, fast-spiking, receptor activation, feedback inhibition, gabaergic, neurons, pyramidal neurons, rhythmic activity, h-current, interneurons, Neurosciences & Neurology
Ämneskategorier Neurovetenskaper

Sammanfattning

Gamma oscillations (30-80 Hz) are fast network activity patterns frequently linked to cognition. They are commonly studied in hippocampal brain slices in vitro, where they can be evoked via pharmacological activation of various receptor families. One limitation of this approach is that neuronal activity is studied in a highly artificial extracellular fluid environment, as provided by artificial cerebrospinal fluid (aCSF). Here, we examine the influence of human cerebrospinal fluid (hCSF) on kainate-evoked and spontaneous gamma oscillations in mouse hippocampus. We show that hCSF, as compared to aCSF of matched electrolyte and glucose composition, increases the power of kainate-evoked gamma oscillations and induces spontaneous gamma activity in areas CA3 and CA1 that is reversed by washout. Bath application of atropine entirely abolished hCSF-induced gamma oscillations, indicating critical contribution from muscarinic acetylcholine receptor-mediated signaling. In separate whole-cell patch clamp recordings from rat hippocampus, hCSF increased theta resonance frequency and strength in pyramidal cells along with enhancement of h-current (I-h) amplitude. We found no evidence of intrinsic gamma frequency resonance at baseline (aCSF) among fast-spiking interneurons, and this was not altered by hCSF. However, hCSF increased the excitability of fast-spiking interneurons, which likely contributed to gamma rhythmogenesis. Our findings show that hCSF promotes network gamma oscillations in the hippocampus in vitro and suggest that neuromodulators distributed in CSF could have significant influence on neuronal network activity in vivo.

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