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Bacterial species associated with persistent apical periodontitis exert differential effects on osteogenic differentiation

Artikel i vetenskaplig tidskrift
Författare A. T. Chow
S. Y. Quah
Gunnar Bergenholtz
K. C. Lim
V. S. H. Yu
K. S. Tan
Publicerad i International Endodontic Journal
Volym 52
Nummer/häfte 2
Sidor 201-210
ISSN 0143-2885
Publiceringsår 2019
Publicerad vid Institutionen för odontologi
Sidor 201-210
Språk en
Länkar dx.doi.org/10.1111/iej.12994
Ämnesord Fusobacterium nucleatum, inflammation, macrophages, osteoblasts, persistent apical periodontitis, necrosis-factor-alpha, canal-treated teeth, alkaline-phosphatase, activity, root-filled teeth, bone-formation, deoxyribonucleic-acid, periapical lesions, collagen-synthesis, interleukin-1-beta, osteoblast
Ämneskategorier Endodonti

Sammanfattning

AimTo determine if bacteria associated with persistent apical periodontitis induce species-specific pro-inflammatory cytokine responses in macrophages, and the effects of this species-specific microenvironment on osteogenic differentiation. MethodologyMacrophages were exposed to Enterococcus faecalis, Streptococcus oralis, Streptococcus mitis, Fusobacterium nucleatum, Treponema denticola or Tannerella forsythia, and levels of TNF- and IL-1 elicited were determined by immunoassay. Following treatment of MG-63 pre-osteoblasts with conditioned media from bacteria-exposed macrophages, osteogenic differentiation and viability of osteoblasts were analyzed by Alizarin Red Staining and MTS assay, respectively. Statistical analysis was carried out by one-way anova with the Tukey post-hoc test. Differences were considered to be significant if P<0.05. ResultsMacrophages exposed to Gram-positive bacteria did not produce significant amounts of cytokines. F.nucleatum-challenged macrophages produced up to four-fold more TNF- and IL-1 compared to T.denticola or T.forsythia. Only conditioned media from macrophages treated with Gram-negative bacteria decreased mineralization and viability of osteoblasts. ConclusionsGram-positive bacteria did not impact osteogenic differentiation and appeared innocuous. Gram-negative bacteria, in particular F.nucleatum elicited an enhanced pro-inflammatory response in macrophages, inhibited osteogenic differentiation and reduced cell viability. The findings suggest that the presence of this organism could potentially increase the severity of persistent apical periodontitis.

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