Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän
Utskriftsversion

A CRISP(e)R view on kidne… - Göteborgs universitet Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

A CRISP(e)R view on kidney organoids allows generation of an induced pluripotent stem cell-derived kidney model for drug discovery

Artikel i vetenskaplig tidskrift
Författare C. Borestrom
A. Jonebring
J. Guo
H. Palmgren
L. Cederblad
A. Forslow
A. Svensson
M. Soderberg
A. Reznichenko
Jenny Nyström
J. Patrakka
R. Hicks
M. Maresca
B. Valastro
A. Collen
Publicerad i Kidney International
Volym 94
Nummer/häfte 6
Sidor 1099-1110
ISSN 0085-2538
Publiceringsår 2018
Publicerad vid Institutionen för neurovetenskap och fysiologi
Sidor 1099-1110
Språk en
Länkar dx.doi.org/10.1016/j.kint.2018.05.0...
Ämnesord glomerulus, kidney development, podocyte, proximal tubule, stem cell, rna-seq, epithelial-cells, podocytes, specificity, injury, cas9, differentiation, proteinuria, mechanisms, crosstalk, Urology & Nephrology
Ämneskategorier Invärtesmedicin

Sammanfattning

Development of physiologically relevant cellular models with strong translatability to human pathophysiology is critical for identification and validation of novel therapeutic targets. Herein we describe a detailed protocol for generation of an advanced 3-dimensional kidney cellular model using induced pluripotent stem cells, where differentiation and maturation of kidney progenitors and podocytes can be monitored in live cells due to CRISPR/Cas9-mediated fluorescent tagging of kidney lineage markers (SIX2 and NPHS1). Utilizing these cell lines, we have refined the previously published procedures to generate a new, higher throughput protocol suitable for drug discovery. Using paraffin-embedded sectioning and whole-mount immunostaining, we demonstrated that organoids grown in suspension culture express key markers of kidney biology (WT1, ECAD, LTL, nephrin) and vasculature (CD31) within renal cortical structures with microvilli, tight junctions and podocyte foot processes visualized by electron microscopy. Additionally, the organoids resemble the adult kidney transcriptomics profile, thereby strengthening the translatability of our in vitro model. Thus, development of human nephron-like structures in vitro fills a major gap in our ability to assess the effect of potential treatment on key kidney structures, opening up a wide range of possibilities to improve clinical translation.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?