Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän
Utskriftsversion

Implant-associated gene e… - Göteborgs universitet Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

Implant-associated gene expression in the jaw bone of smokers and nonsmokers: A human study using quantitative qPCR

Artikel i vetenskaplig tidskrift
Författare Shariel Sayardoust
Omar Omar
O. Norderyd
Peter Thomsen
Publicerad i Clinical Oral Implants Research
Volym 29
Nummer/häfte 9
Sidor 937-953
ISSN 0905-7161
Publiceringsår 2018
Publicerad vid Institutionen för kliniska vetenskaper, Avdelningen för biomaterialvetenskap
Sidor 937-953
Språk en
Länkar dx.doi.org/10.1111/clr.13351
Ämnesord bone-implant interactions, clinical research, clinical trials, genetics, host mechanisms, material, necrosis-factor-alpha, oxidized titanium implants, osteoblast, differentiation, endosseous implants, adherent cells, oral implants, blood-flow, follow-up, in-vivo, osseointegration, Dentistry, Oral Surgery & Medicine, Engineering
Ämneskategorier Biomaterialvetenskap

Sammanfattning

ObjectivesThis study aimed to compare the molecular events in implant-adherent cells and in peri-implant bone during the osseointegration of machined and oxidized titanium implants in smokers and nonsmokers. Materials and MethodsTwenty-four smokers and 24 nonsmokers each received machined and anodically oxidized mini-implants. The mini-implants and the surrounding bone were retrieved after 1, 7, and 28days, for gene expression analysis of selected factors using quantitative polymerase chain reaction (qPCR). ResultsDifferences between machined and oxidized implants were more evident in the implant-adherent cells than the peri-implant bone. The machined implants revealed higher expression of proinflammatory cytokines, interleukin-8 (IL-8) (in nonsmokers), and tumor necrosis factor-alpha (in nonsmokers and smokers), compared with the oxidized implants. Conversely, the expression of bone formation genes, alkaline phosphatase and osteocalcin, was generally higher at the oxidized implants. In smokers, the temporal pattern revealed the delayed and initial inhibition of osteoblastic and osteoclastic gene expression, respectively, mainly at the machined implants. In contrast, oxidized implants revealed higher expression of bone remodeling, cathepsin K (CatK) and calcitonin receptor, and coupling, receptor activator of nuclear factor kappa-B ligand (RANKL) and osteoprotegerin, genes after 7day in smokers. ConclusionsThe implant-adherent cells are more sensitive to surface properties and smoking conditions than the cells in the peri-implant bone. Smoking imposes inhibitory effects on the initial molecular events of osseointegration in the human bone-implant interface. The surface properties of oxidized implants appear to have a beneficial effect on osseointegration by mitigating the smoking-induced negative effects.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?