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The extracellular matrix-degrading protein ADAMTS5 is expressed in the nuclei of urothelial cells in healthy rats

Artikel i vetenskaplig tidskrift
Författare D. Delbro
Lena Hallsberg
Ralph Peeker
N. Scherbak
Magnus Fall
Rebecka Arnsrud Godtman
Publicerad i Scandinavian Journal of Urology
Volym 52
Nummer/häfte 2
Sidor 139-142
ISSN 2168-1805
Publiceringsår 2018
Publicerad vid Institutionen för kliniska vetenskaper, Avdelningen för kirurgi
Institutionen för kliniska vetenskaper, Avdelningen för urologi
Sidor 139-142
Språk en
Länkar dx.doi.org/10.1080/21681805.2017.14...
Ämnesord ADAMTS5, aggrecanase, confocal laser scanning microscopy, immunohistochemistry, nuclear, rat urothelium, Urology & Nephrology
Ämneskategorier Urologi och njurmedicin

Sammanfattning

Objective: The aim of this study was to investigate whether protein expression of the extracellular matrix-degrading protease ADAMTS5 can be demonstrated in the urinary bladder of healthy rats, and, if so, to determine the localization of this enzyme. Materials and methods: The experiments were conducted with eight inbred male Sprague-Dawley rats. Immunohistochemistry was used to investigate the expression of ADAMTS5 in the urinary bladder. Negative controls were established by either excluding the primary antibody or applying the antibody after it had been preabsorbed with its immunogenic peptide. Confocal microscopy was used to visualize the distribution of ADAMTS5 in the urinary bladder tissue. Results: Immunoreactivity for ADAMTS5 was demonstrated in the urothelium and in the detrusor. This expression was localized not only in the cytoplasm, but also in the nuclei. Confocal microscopy corroborated these findings. Conclusion: Expression of ADAMTS5 was demonstrated in the cytoplasm as well as in the nuclei of the urothelium and detrusor cells, suggesting that it may play a role at the transcriptional level.

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