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Micropatterned Scaffolds with Immobilized Growth Factor Genes Regenerate Bone and Periodontal Ligament-Like Tissues.

Artikel i vetenskaplig tidskrift
Författare Sophia P Pilipchuk
Tobias Fretwurst
Ning Yu
Lena Larsson
Nolan M Kavanagh
Farah Asa'ad
Kenneth C K Cheng
Joerg Lahann
William V Giannobile
Publicerad i Advanced healthcare materials
Volym 7
Nummer/häfte 22
Sidor e1800750
ISSN 2192-2659
Publiceringsår 2018
Publicerad vid Institutionen för odontologi
Sidor e1800750
Språk en
Länkar dx.doi.org/10.1002/adhm.201800750
www.ncbi.nlm.nih.gov/entrez/query.f...
Ämneskategorier Odontologi

Sammanfattning

Periodontal disease destroys supporting structures of teeth. However, tissue engineering strategies offer potential to enhance regeneration. Here, the strategies of patterned topography, spatiotemporally controlled growth factor gene delivery, and cell-based therapy to repair bone-periodontal ligament (PDL) interfaces are combined. Micropatterned scaffolds are fabricated for the ligament regions using polycaprolactone (PCL)/polylactic-co-glycolic acid and combined with amorphous PCL scaffolds for the bone region. Scaffolds are modified using chemical vapor deposition, followed by spatially controlled immobilization of vectors encoding either platelet-derived growth factor-BB or bone morphogenetic protein-7, respectively. The scaffolds are seeded with human cells and delivered to large alveolar bone defects in athymic rats. The effects of dual and single gene delivery with and without micropatterning are assessed after 3, 6, and 9 weeks. Gene delivery results in greater bone formation at three weeks. Micropatterning results in regenerated ligamentous tissues similar to native PDL. The combination results in more mature expression of collagen III and periostin, and with elastic moduli of regenerated tissues that are statistically indistinguishable from those of native tissue, while controls are less stiff than native tissues. Thus, controlled scaffold microtopography combined with localized growth factor gene delivery improves the regeneration of periodontal bone-PDL interfaces.

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