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SECAPR-a bioinformatics pipeline for the rapid and user-friendly processing of targeted enriched Illumina sequences, from raw reads to alignments

Artikel i vetenskaplig tidskrift
Författare Tobias Andermann
A. Cano
Alexander Zizka
Christine D. Bacon
Alexandre Antonelli
Publicerad i Peerj
Volym 6
ISSN 2167-8359
Publiceringsår 2018
Publicerad vid Institutionen för biologi och miljövetenskap
Språk en
Länkar dx.doi.org/10.7717/peerj.5175
Ämnesord Next generation sequencing (NGS), Exon capture, FASTQ, Contig, Allele phasing, Phylogenetics, ultraconserved elements, generation, genome, Science & Technology - Other Topics
Ämneskategorier Miljövetenskap

Sammanfattning

Evolutionary biology has entered an era of unprecedented amounts of DNA sequence data, as new sequencing technologies such as Massive Parallel Sequencing (MPS) can generate billions of nucleotides within less than a day. The current bottleneck is how to efficiently handle, process, and analyze such large amounts of data in an automated and reproducible way. To tackle these challenges we introduce the Sequence Capture Processor (SECAPR) pipeline for processing raw sequencing data into multiple sequence alignments for downstream phylogenetic and phylogeographic analyses. SECAPR is user-friendly and we provide an exhaustive empirical data tutorial intended for users with no prior experience with analyzing MPS output. SECAPR is particularly useful for the processing of sequence capture (synonyms: target or hybrid enrichment) datasets for non-model organisms, as we demonstrate using an empirical sequence capture dataset of the palm genus Geonoma (Arecaceae). Various quality control and plotting functions help the user to decide on the most suitable settings for even challenging datasets. SECAPR is an easy-to-use, free, and versatile pipeline, aimed to enable efficient and reproducible processing of MPS data for many samples in parallel.

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