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High-resolution quantitative proteome analysis reveals substantial differences between phagosomes of RAW 264.7 and bone marrow derived macrophages.

Artikel i vetenskaplig tidskrift
Författare Manman Guo
Anetta Härtlova
Brian D Dill
Alan R Prescott
Marek Gierliński
Matthias Trost
Publicerad i Proteomics
Volym 15
Nummer/häfte 18
Sidor 3169-74
ISSN 1615-9861
Publiceringsår 2015
Publicerad vid
Sidor 3169-74
Språk en
Länkar dx.doi.org/10.1002/pmic.201400431
www.ncbi.nlm.nih.gov/entrez/query.f...
Ämnesord Animals, Cells, Cultured, Databases, Protein, Macrophages, chemistry, Mice, Phagosomes, chemistry, Protein Interaction Maps, Proteome, analysis, chemistry, classification, Proteomics, RAW 264.7 Cells, chemistry, cytology
Ämneskategorier Biokemi och molekylärbiologi, Immunologi

Sammanfattning

Macrophages are important immune cells operating at the forefront of innate immunity by taking up foreign particles and microbes through phagocytosis. The RAW 264.7 cell line is commonly used for experiments in the macrophage and phagocytosis field. However, little is known how its functions compare to primary macrophages. Here, we have performed an in-depth proteomics characterization of phagosomes from RAW 264.7 and bone marrow derived macrophages by quantifying more than 2500 phagosomal proteins. Our data indicate that there are significant differences for a large number of proteins including important receptors such as mannose receptor 1 and Siglec-1. Moreover, bone marrow derived macrophages phagosomes mature considerably faster by fusion with endosomes and the lysosome which we validated using fluorogenic phagocytic assays. We provide a valuable resource for researcher in the field and recommend careful use of the RAW 264.7 cell line when studying phagosome functions. All MS data have been deposited in the ProteomeXchange with identifier PXD001293 (http://proteomecentral.proteomexchange.org/dataset/PXD001293).

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