Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän
Utskriftsversion

Granule-stored MUC5B muci… - Göteborgs universitet Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

Granule-stored MUC5B mucins are packed by the non-covalent formation of N-terminal head-to-head tetramers

Artikel i vetenskaplig tidskrift
Författare Sergio Trillo-Muyo
Harriet E. Nilsson
Christian V Recktenwald
Anna Ermund
C. Ridley
Lauren N Meiss
A. Bahr
N. Klymiuk
Jeffrey J. Wine
P. J. B. Koeck
D. J. Thornton
H. Hebert
Gunnar C. Hansson
Publicerad i Journal of Biological Chemistry
Volym 293
Nummer/häfte 15
Sidor 5746-5754
ISSN 0021-9258
Publiceringsår 2018
Publicerad vid Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi
Sidor 5746-5754
Språk en
Länkar doi.org/10.1074/jbc.RA117.001014
Ämnesord von-willebrand-factor, gel-forming-mucin, cystic-fibrosis, secretion, release, mucus, bicarbonate, packing, tubules, domain, Biochemistry & Molecular Biology
Ämneskategorier Biokemi och molekylärbiologi

Sammanfattning

Most MUC5B mucin polymers in the upper airways of humans and pigs are produced by submucosal glands. MUC5B forms N-terminal covalent dimers that are further packed into larger assemblies because of low pH and high Ca2+ in the secretory granule of the mucin-producing cell. We purified the recombinant MUC5B N-terminal covalent dimer and used single-particle electron microscopy to study its structure under intracellular conditions. We found that, at intragranular pH, the dimeric MUC5B organized into head-to-head noncovalent tetramers where the von Willebrand D1-D2 domains hooked into each other. These N-terminal tetramers further formed long linear complexes from which, we suggest, the mucin domains and their C termini project radially outwards. Using conventional and video microscopy, we observed that, upon secretion into the submucosal gland ducts, a flow of bicarbonate-rich fluid passes the mucin-secreting cells. We suggest that this unfolds and pulls out the MUC5B assemblies into long linear threads. These further assemble into thicker mucin bundles in the glandular ducts before emerging at the gland duct opening. We conclude that the combination of intracellular packing of the MUC5B mucin and the submucosal gland morphology creates an efficient machine for producing linear mucin bundles.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?