Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän
Utskriftsversion

Assessing antigen specifi… - Göteborgs universitet Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

Assessing antigen specific HLA-DR plus antibody secreting cell (DR plus ASC) responses in whole blood in enteric infections using an ELISPOT technique

Artikel i vetenskaplig tidskrift
Författare T. R. Bhuiyan
M. R. Hoq
N. S. Nishat
D. Al Mahbuba
R. Rashu
K. Islam
L. Hossain
J. B. Harris
E. T. Ryan
S. B. Calderwood
Ann-Mari Svennerholm
F. Qadri
Publicerad i Microbes and Infection
Volym 20
Nummer/häfte 2
Sidor 122-129
ISSN 1286-4579
Publiceringsår 2018
Publicerad vid Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi
Sidor 122-129
Språk en
Länkar doi.org/10.1016/j.micinf.2017.10.00...
https://gup.ub.gu.se/file/207319
Ämnesord HLA-DR, ASCs, Immune response, Cholera, ETEC, ELISPOT, enterotoxigenic escherichia-coli, colonization factors, immune-responses, monoclonal-antibodies, travelers diarrhea, forming-cells, global burden, expression, molecules, cholera
Ämneskategorier Infektionsmedicin, Mikrobiologi

Sammanfattning

Antibody secreting cells (ASCs) generate antibodies in an antigen-specific manner as part of the adaptive immune response to infections, and these cells increase their surface expression of HLA-DR. We have studied this parameter (HLA-DR+ASC) in patients with recent diarrheal infection using immuno-magnetic cell sorting and an enzyme linked immunospot (ELISPOT) technique that requires only one milliliter of blood. We validated this approach in adult patients with cholera (n = 15) or ETEC diarrhea (n = 30) on days 2, 7 and 30 after showing clinical symptom at the International Centre for Diarrhoeal Disease Research, Bangladesh (icddr, b) hospital in Dhaka, and we compared responses to age-matched healthy controls (n = 7). We found that HLA-DRthorn ASC (DR+ASC) responses specific both for T cell-dependent (cholera toxin B subunit), and T cell-independent (lipopolysaccharide) antigens were elevated at day 7 after showing clinical cholera symptom. Similarly, DR+ASCs were elevated against both heat-labile toxin and colonization factors following ETEC infection. We observed significant correlations between antigen-specific DR+ASC responses and antigen-specific, gut homing ASC and plasma antibody responses. This study demonstrates that a simple ELISPOT procedure allows determination of antigen-specific ASC responses using a small volume of whole blood following diarrhea. This technique may be particularly useful in studying DR+ASC responses in young children and infants, either following infection or vaccination. (c) 2017 The Authors. Published by Elsevier Masson SAS on behalf of Institut Pasteur.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?