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Luminal solutions protect mucosal barrier during extended preservation.

Artikel i vetenskaplig tidskrift
Författare Mihai Oltean
Mats Hellström
Catalin Ciuce
Changlian Zhu
Anna Casselbrant
Publicerad i The Journal of surgical research
Volym 194
Nummer/häfte 1
Sidor 289-96
ISSN 1095-8673
Publiceringsår 2015
Publicerad vid Institutionen för kliniska vetenskaper, Avdelningen för kirurgi
Institutionen för kliniska vetenskaper, Avdelningen för obstetrik och gynekologi
Sidor 289-96
Språk en
Länkar dx.doi.org/10.1016/j.jss.2014.10.00...
www.ncbi.nlm.nih.gov/entrez/query.f...
Ämnesord Animals, Apoptosis, Caspase 3, metabolism, Glutamine, pharmacology, Goblet Cells, pathology, Intestinal Mucosa, metabolism, pathology, Intestines, transplantation, Male, Organ Preservation, Polyethylene Glycols, pharmacology, Rats, Rats, Sprague-Dawley, Solutions, Tight Junctions, physiology
Ämneskategorier Medicinska grundvetenskaper

Sammanfattning

Mucosal barrier injury during intestinal preservation (IP) and transplantation favors life-threatening infections. Luminal delivery of solutions containing amino acids or polyethylene glycols (PEGs) may improve preservation results and reduce this injury. We tested if solutions containing glutamine and PEG influence the mucosal injury.Rat intestines were perfused and stored in Viaspan-University of Wisconsin solution. Before IP, a PEG 3350 solution was introduced intraluminally alone (group 1) or supplemented with 40 mmol/L L-glutamine (group 2). Controls underwent vascular flush alone (group 3). Preservation injury was evaluated after 8, 14, and 24 h by histology and goblet cell count. Tight-junction proteins zonula occludens-1, claudin-3, claudin-4, and caveolin-1 were studied by immunofluorescence. Maltase and caspase-3 activity were also analyzed.Group 1 showed mild edema at 8 h and mucosal disruption by 24 h; these features were greatly improved in group 2 where continuous mucosa was found after 24 h of IP. Intestines in group 3 did worse at all time points with subepithelial edema (Park/Chiu grade 3) and marked goblet cell depletion; caspase-3 activity was lowest in group 2. Tight-junction proteins varied continuously during IP; zonula occludens-1 expression and colocalization with claudins decreased significantly in group 3 but not in other groups. Claudin-3 was distinctly localized in the membrane, but stained diffuse, cytoplasmic at later time-points. Claudin-4 changed to a cytoplasmic granular pattern. No caveolin-1 colocalization was observed.Luminal PEG and glutamine delay epithelial breakdown and preserve several important mucosal features during extended IP.

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