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Staphylococcal biofilm gene expression on biomaterials - A methodological study

Artikel i vetenskaplig tidskrift
Författare Annika Juhlin
Sara Svensson
Peter Thomsen
Margarita Trobos
Publicerad i Journal of Biomedical Materials Research Part A
Volym 105
Nummer/häfte 12
Sidor 3400-3412
ISSN 1549-3296
Publiceringsår 2017
Publicerad vid Institutionen för kliniska vetenskaper, Avdelningen för biomaterialvetenskap
Sidor 3400-3412
Språk en
Länkar doi.org/10.1002/jbm.a.36171
Ämnesord biofilm, staphylococci, implant surface, gene expression, RT-qPCR, phenol-soluble modulins, aureus biofilms, epidermidis biofilms, quantitative pcr, clumping factor, in-vitro, infections, lysostaphin, mechanisms, resistance, Engineering, Materials Science, hindler ca, 1964, proceedings of the national academy of sciences of the united states of america, v51, p414
Ämneskategorier Biomaterialvetenskap

Sammanfattning

The combination of increased healthcare access, universal aging, and infallible therapy demands, synergistically drive the need for the development of biomaterial technologies that mitigate the challenge of biomaterial-associated infections (BAI). Staphylococcus epidermidis and Staphylococcus aureus account for the majority of BAI due to their ability to accumulate in adherent multilayered biofilm. This investigation details the development of gene expression assays to evaluate the genetic processes of attachment, accumulation, maturation, and dispersal phases of biofilms on biomaterials in vitro, while abiding by the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines. The biofilm formation of S. epidermidis on polyurethane (PU) central venous catheters and S. aureus on machined titanium (Ti) was examined in terms of gene expression at early and late time points. The results provided insight into how each stage of biofilm formation is orchestrated over time on these biomaterials in vitro. Furthermore, the results suggested that mechanical RNA extraction, organic solvents, elimination of genomic DNA, and preamplification are advisable strategies to implement for biofilm gene expression analysis. It is concluded that this method can be employed for the assessment of biofilm-biomaterial interactions at the molecular level. (c) 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3400-3412, 2017.

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