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Influence of Glycosylation on Interfacial Properties of Recombinant Mucins: Adsorption, Surface Forces, and Friction

Artikel i vetenskaplig tidskrift
Författare J. X. An
Chunsheng Jin
A. Dedinaite
Jan Holgersson
Niclas G. Karlsson
P. M. Claesson
Publicerad i Langmuir
Volym 33
Nummer/häfte 18
Sidor 4386-4395
ISSN 0743-7463
Publiceringsår 2017
Publicerad vid Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin
Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi
Sidor 4386-4395
Språk en
Länkar doi.org/10.1021/acs.langmuir.7b0003...
Ämnesord bovine submaxillary mucin, porcine gastric mucin, hydrophobic surfaces, fusion proteins, microscope cantilevers, boundary lubrication, polymer, oligosaccharides, glycoprotein, lubricin
Ämneskategorier Klinisk laboratoriemedicin

Sammanfattning

Interfacial properties of two brush-with-anchor mucins, C-P55 and C-PSLex, have been investigated at the aqueous solution/poly(methyl methacrylate) (PMMA) interface. Both are recombinant mucin-type fusion proteins, produced by fusing the glycosylated mucin part of P-selectin glycoprotein ligand-1 (PSLG-1) to the Fc part of a mouse immunoglobulin in two different cells. They are mainly expressed as dimers upon production. Analysis of the O-glycans shows that the C-PSLex mucin has the longer and more branched side chains, but C-P55 has slightly higher sialic acid content. The adsorption of the mucins to PMMA surfaces was studied by quartz crystal microbalance with dissipation. The sensed mass, including the adsorbed mucin and water trapped in the layer, was found to be similar for these two mucin layers. Atomic force microscopy with colloidal probe was employed to study surface and friction forces between mucin-coated PMMA surfaces. Purely repulsive forces of steric origin were observed between mucin layers on compression, whereas a small adhesion was detected between both mucin layers on decompression. This was attributed to chain entanglement. The friction force between C-PSLex-coated PMMA is lower than that between C-P55-coated PMMA. at low loads, but vice versa at high loads. We discuss our results in terms of the differences in the glycosylation composition of these two mucins.

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