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Biofilm formation and antimicrobial susceptibility of staphylococci and enterococci from osteomyelitis associated with percutaneous orthopaedic implants.

Artikel i vetenskaplig tidskrift
Författare Magdalena Zaborowska
Jonatan Tillander
Rickard Brånemark
Lars Hagberg
Peter Thomsen
Margarita Trobos
Publicerad i Journal of biomedical materials research. Part B, Applied biomaterials
Volym 105
Nummer/häfte 8
Sidor 2630–2640
ISSN 1552-4981
Publiceringsår 2017
Publicerad vid Institutionen för kliniska vetenskaper, Avdelningen för biomaterialvetenskap
Institutionen för biomedicin, avdelningen för infektionssjukdomar
Institutionen för kliniska vetenskaper, Avdelningen för ortopedi
Sidor 2630–2640
Språk en
Länkar dx.doi.org/10.1002/jbm.b.33803
www.ncbi.nlm.nih.gov/entrez/query.f...
Ämnesord Enterococcus; MBEC; MIC; Staphylococcus; biofilm; biomaterial-associated infections
Ämneskategorier Biomaterialvetenskap, Ortopedi

Sammanfattning

Staphylococci and enterococci account for most deep infections associated with bone-anchored percutaneous implants for amputation treatment. Implant-associated infections are difficult to treat; therefore, it is important to investigate if these infections have a biofilm origin and to determine the biofilm antimicrobial susceptibility to improve treatment strategies. The aims were: (i) to test a novel combination of the Calgary biofilm device and a custom-made susceptibility MIC plate (Sensititre(®) ), (ii) to determine the biofilm formation and antimicrobial resistance in clinical isolates causing implant-associated osteomyelitis, and (iii) to describe the associated clinical outcome. Enterococci and staphylococci were characterized by microtitre plate assay, Congo Red Agar plate test, and PCR. Biofilm susceptibility to 10 antimicrobials and its relationship to treatment outcomes were determined. The majority of the strains produced biofilm in vitro showing inter- and intraspecies differences. Biofilms showed a significantly increased antimicrobial resistance compared with their planktonic counterparts. Slime-producing strains tolerated significantly higher antimicrobial concentrations compared with non-producers. All seven staphylococcal strains carried ica genes, but two did not produce slime. The degree of biofilm formation and up-regulated antibiotic resistance may translate into a variable risk of treatment failure. This new method set-up allows for the reproducible determination of minimum biofilm eradication concentration of antimicrobial agents, which may guide future antimicrobial treatment decisions in orthopaedic implant-associated infection. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2016.

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